Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, PR China.
Res Vet Sci. 2012 Aug;93(1):366-73. doi: 10.1016/j.rvsc.2011.06.020. Epub 2011 Jul 20.
Lactobacillus reuteri ATCC 55730 (L. reuteri ATCC 55730) and L. reuteri L22 were studied for their probiotic potential. These two strains were able to produce an antimicrobial substance, termed reuterin, the maximum production of reuterin by these two strains was detected in the late logarithmic growth phase (16 h in MRS and 20 h in LB broths). These two strains could significantly reduce the growth of Salmonella pullorum ATCC 9120 in MRS broth, L. reuteri ATCC 55730 with a reduction of 48.2±4.15% (in 5 log) and 89.7±2.59% (in 4 log) respectively, at the same time, L. reuteri L22 was 69.4±3.48% (in 5 log) and 80.4±3.22% respectively. L. reuteri ATCC 55730 was active against the majority of the pathogenic species, including S. pullorum ATCC 9120 and Escherichia coli O(78), while L. reuteri L22 was not as effective as L. reuteri ATCC 55730. The two potential strains were found to survive variably at pH 2.5 and were unaffected by bile salts, while neither of the strains was haemolytic. Moreover, L. reuteri ATCC 55730 exhibited variable susceptibility towards commonly used antibiotics; but L. reuteri L22 showed resistant to most antibiotics in this study. L. reuteri ATCC 55730 consequently was found to significantly increase survival rate in a Salmonella-induced pullorum disease model in chick. To conclude, strain L. reuteri ATCC 55730 possesses desirable probiotic properties, such as antimicrobial activity and immunomodulation in vitro, which were confirmed in vivo by the use of animal models.
研究了乳杆菌雷特氏菌 ATCC 55730（L. reuteri ATCC 55730）和 L. reuteri L22 的益生菌潜力。这两种菌株能够产生一种抗菌物质，称为雷替丁，这两种菌株的雷替丁最大产量是在对数生长期晚期（MRS 肉汤中为 16 小时，LB 肉汤中为 20 小时）检测到的。这两种菌株可以显著降低 MRS 肉汤中鸡白痢沙门氏菌 ATCC 9120 的生长，L. reuteri ATCC 55730 的减少分别为 48.2±4.15%（5 对数）和 89.7±2.59%（4 对数），同时，L. reuteri L22 的减少分别为 69.4±3.48%（5 对数）和 80.4±3.22%。L. reuteri ATCC 55730 对大多数致病性物种有效，包括鸡白痢沙门氏菌 ATCC 9120 和大肠杆菌 O(78)，而 L. reuteri L22 不如 L. reuteri ATCC 55730 有效。这两种潜在的菌株在 pH 2.5 下的存活率不同，不受胆汁盐的影响，而且两种菌株都没有溶血作用。此外，L. reuteri ATCC 55730 对常用抗生素的敏感性不同；但 L. reuteri L22 在本研究中对大多数抗生素均表现出耐药性。L. reuteri ATCC 55730 因此在鸡白痢沙门氏菌诱导的雏鸡疾病模型中显著提高了存活率。总之，L. reuteri ATCC 55730 菌株具有理想的益生菌特性，如体外的抗菌活性和免疫调节作用，这些特性在动物模型中得到了证实。
