1 Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
2 Research and development center, Betagro group, 136 Moo 9, Klong Nueng, Klong Luang Pathumthani 12120, Thailand.
Benef Microbes. 2019 Feb 8;10(1):43-54. doi: 10.3920/BM2018.0034. Epub 2018 Nov 8.
Poultry is an important high-quality food and protein source for humans. However, chicken is considered a primary source of foodborne diseases, especially Salmonella Enteritidis infection. Reducing Salmonella contamination in live poultry will thus lower the risk to consumers. Our previous studies reported that Lactobacillus reuteri KUB-AC5 can produce a substance with antimicrobial activity against pathogenic bacteria, especially Salmonella. In vivo testing revealed that this strain greatly influenced the ileal microbiota by improving chicken gastrointestinal health and inhibiting certain pathogenic bacteria. However, its activity against Salmonella in chicken is unknown. This study investigated the effects of the probiotic L. reuteri KUB-AC5 at various concentrations against Salmonella and the microbiota status in the gastrointestinal tract of broiler chickens. Four treatments groups were used: negative-control group (no Salmonella challenge), positive-control group (Salmonella challenge), and 5 or 7 log cfu probiotic supplementation to Salmonella-challenged chickens. The resultant microbial diversities at the growing and finisher stages were not significantly different among the groups (P>0.05). However, a high dosage of KUB-AC5 maintained similar microbial diversity in Salmonella-challenged chickens as observed in the non-challenged group in the early stage. The exposure Salmonella can affect the microbial diversity that consequently contributes to the disease progression in chicken. Low and high dosages of KUB-AC5 eliminated S. Enteritidis from the ileum and caecum at 14, 21 and 35 days of age. A high-dose of KUB-AC5 also enhanced Lactobacillaceae levels in the growing stage in both the ileum and caecum and suppressed Enterobacteriaceae levels in the finisher stage on day 35, whereas these effects were not observed in the low dose of KUB-AC5 or control groups. These results support the potential value of high-dose L. reuteri KUB-AC5 supplementation for three days after hatching in preventing Salmonella infection in chickens.
家禽是人类重要的优质食品和蛋白质来源。然而，鸡被认为是食源性疾病的主要来源，尤其是肠炎沙门氏菌感染。因此，减少活禽中的沙门氏菌污染将降低消费者的风险。我们之前的研究报告称，鼠李糖乳杆菌 KUB-AC5 可以产生一种对致病菌，特别是沙门氏菌具有抗菌活性的物质。体内试验表明，该菌株通过改善鸡的胃肠道健康和抑制某些致病菌，对鸡的回肠微生物群有很大影响。然而，其在鸡中对沙门氏菌的活性尚不清楚。本研究调查了不同浓度的益生菌鼠李糖乳杆菌 KUB-AC5 对沙门氏菌的影响以及肉鸡胃肠道微生物群的状态。使用了四个处理组：阴性对照组（无沙门氏菌挑战）、阳性对照组（沙门氏菌挑战）和 5 或 7 log cfu 益生菌补充到沙门氏菌挑战的鸡中。在生长和育肥阶段，各组的微生物多样性没有显著差异（P＞0.05）。然而，高剂量的 KUB-AC5 在早期保持了与非挑战组相似的微生物多样性沙门氏菌挑战组的鸡。暴露于沙门氏菌会影响微生物多样性，从而导致鸡的疾病进展。低剂量和高剂量的 KUB-AC5 可在 14、21 和 35 日龄时从回肠和盲肠中消除肠炎沙门氏菌。高剂量的 KUB-AC5 还增强了生长阶段回肠和盲肠中乳杆菌科的水平，并抑制了育肥阶段第 35 天肠杆菌科的水平，而低剂量的 KUB-AC5 或对照组则没有观察到这些效果。这些结果支持高剂量鼠李糖乳杆菌 KUB-AC5 补充剂在孵化后三天内补充的潜在价值，可预防鸡的沙门氏菌感染。
