Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University Giessen, Giessen, Germany.
Res Vet Sci. 2012 Aug;93(1):321-30. doi: 10.1016/j.rvsc.2011.06.010. Epub 2011 Jul 20.
The aim of this prospective study was to characterize the response of the coagulation system to a defined sterile localized inflammatory process. Tissue cages were implanted subcutaneously in five healthy Beagles. After 9-10 weeks, local inflammation was induced by an injection of 0.5 ml 1% carrageenan. Serial samples of tissue cage fluid (TCF) and blood were collected at 10 time points (0-168 h). Nucleated cells (NC) of TCF were counted automatically to characterize local inflammation. C-reactive protein (CRP), leukocytes and coagulation variables (PT, aPTT, fibrinogen, factor VIII, antithrombin, protein C, protein S, and d-dimers) were determined in blood samples. Carrageenan induced a significant 32-fold increase of NCs in TCF (P<0.0001). A slight increase in leukocytes (P<0.0001) was observed. There was a significant 1.3- to 1.5-fold increase in protein C (P=0.0001) and protein S (P=0.0028). CRP, secondary hemostasis and fibrinolysis did not change. The mild increase from baseline in PC/PS, may reflect a physiological counter reaction.
本前瞻性研究旨在描述凝血系统对特定无菌局部炎症反应的应答特征。将组织笼植入五只健康比格犬的皮下。9-10 周后，通过注射 0.5ml1%角叉菜胶诱导局部炎症。在 10 个时间点（0-168 小时）采集组织笼液（TCF）和血液的连续样本。自动计数 TCF 的有核细胞（NC）以表征局部炎症。在血液样本中测定 C 反应蛋白（CRP）、白细胞和凝血变量（PT、aPTT、纤维蛋白原、VIII 因子、抗凝血酶、蛋白 C、蛋白 S 和 D-二聚体）。角叉菜胶诱导 TCF 中的 NC 显著增加 32 倍（P<0.0001）。白细胞略有增加（P<0.0001）。蛋白 C（P=0.0001）和蛋白 S（P=0.0028）增加了 1.3-1.5 倍。CRP、二级止血和纤维溶解没有改变。PC/PS 从基线的轻度增加可能反映了一种生理的代偿反应。
