Mincheva A, Slavchev B, Karag'ozov I, Vasilev N, Makaveeva V
Akush Ginekol (Sofiia). 1990;29(4):41-4.
34 cellular suspensions from the vaginal part of women were examined for presence of HPV DNA of the types: 6, 11, 16 and 18--from a lesion and a healthy area--by filter in situ hybridization. The women were examined by colposcopy because of cervical lesions established macroscopically as well as for other various complaints. Cells were also investigated from women after undergone ablation treatment: diathermy coagulation, conization and hysterectomy on account of CIN. The data were compared with cytological, colposcopic and histological findings. The established presence of HPV DNA of high risk type 16 and 18 in some of the patients after operative treatment required continuous control of these women as well as examination of lower genital tract because of the discovered multicentricity of HPV infection.
通过滤膜原位杂交技术,对34名女性阴道部位的细胞悬液进行检测,以确定是否存在6、11、16和18型人乳头瘤病毒(HPV)DNA,这些样本取自病变部位和健康区域。由于肉眼可见的宫颈病变以及其他各种症状,这些女性接受了阴道镜检查。此外,还对因宫颈上皮内瘤变(CIN)接受过消融治疗(透热凝固、锥形切除术和子宫切除术)的女性的细胞进行了研究。将这些数据与细胞学、阴道镜检查和组织学结果进行了比较。部分患者在接受手术治疗后,检测到高危型16和18型HPV DNA的存在,鉴于发现HPV感染具有多中心性,因此需要对这些女性进行持续监测,并对其下生殖道进行检查。