First Department of Pediatrics, Semmelweis University, Budapest, Bókay u. 53-54, H-1083, Hungary.
J Neuroimmunol. 2011 Aug 15;237(1-2):80-6. doi: 10.1016/j.jneuroim.2011.06.008. Epub 2011 Jul 20.
Kv1.3 and IKCa1 potassium channels play an important role in the maintenance of calcium-influx during lymphocyte activation and present a possible target for selective immunomodulation. We investigated the calcium-influx characteristics of Th1, Th2, CD4, CD8 T-lymphocytes isolated from multiple sclerosis patients without or with interferon-beta therapy, and its modulation by Kv1.3 and IKCa1 channel inhibitors using flow cytometry. Specific immunomodulation of the CD8 subset can be reached through inhibition of Kv1.3 channels in multiple sclerosis patients without interferon-beta. However, this effect is not specific enough concerning all lymphocyte subsets influencing the autoimmune response, since it also affects anti-inflammatory Th2 cells.
钾离子通道 Kv1.3 和 IKCa1 在淋巴细胞激活过程中钙内流的维持中起着重要作用，它们是选择性免疫调节的可能靶点。我们使用流式细胞术研究了多发性硬化症患者（包括未接受干扰素-β治疗和接受干扰素-β治疗的患者）分离的 Th1、Th2、CD4、CD8 T 淋巴细胞的钙内流特征，以及 Kv1.3 和 IKCa1 通道抑制剂对其的调节作用。在未接受干扰素-β治疗的多发性硬化症患者中，通过抑制 Kv1.3 通道可以特异性地调节 CD8 亚群。然而，由于它也会影响抗炎性 Th2 细胞，因此这种作用对于影响自身免疫反应的所有淋巴细胞亚群来说并不够特异。
