Dipartimento di Scienze e Tecnologie Chimiche, Tor Vergata University, Via della Ricerca Scientifica, Rome 00133, Italy.
Bioorg Med Chem. 2011 Aug 15;19(16):5071-8. doi: 10.1016/j.bmc.2011.05.058. Epub 2011 May 31.
A novel multienzyme biocatalyst, based on coimmobilization of the laccase and horseradish peroxidase by cross linking and layer-by-layer coating with polyelectrolyte, was designed, synthesized and applied at the development of an oxidative cascade process on lignin. The efficiency and specificity of the new LbL-multienzyme system, the occurrence of a synergy of the co-immobilized enzymes, the lignin oxidation pathway and the nature of the structural modifications occurred in treated lignins have been investigated in the present effort by means of GPC analysis and quantitative (31)P NMR techniques.
设计、合成了一种新型多酶生物催化剂，通过交联和聚电解质层层涂层将漆酶和辣根过氧化物酶共固定化，应用于木质素的氧化级联过程。本研究采用凝胶渗透色谱分析和定量（31）P NMR 技术，研究了新型 LbL 多酶体系的效率和特异性、共固定化酶的协同作用的发生、木质素的氧化途径以及处理木质素中发生的结构修饰的性质。
