3-羟基邻苯二甲酸酐修饰的卵清蛋白对单纯疱疹病毒2的体外抗病毒活性
[Antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin against herpes simplex virus 2 in vitro].
作者信息
He Li-li, Duan Jiang-man, Qiu Jia-yin, Yu Fei, Liu Shu-wen, Li Lin
机构信息
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(7):1175-8.
OBJECTIVE
To investigate the antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin (HP-OVA) against herpes simplex virus 2 (HSV-2) in vitro.
METHODS
By chemical modification, ovalbumin (OVA) was treated with 3-hydroxyphthalic anhydride (HP) to prepare HP-OVA. The anti-HSV-2 activity against HSV-2 333 virus in vitro and the cytotoxicity of HP-OVA in African green monkey kidney cells (Vero cells) were detected by MTT colorimetric assay. The inhibitory effects of HP-OVA on 17 strains of vaginal lactobacilli were observed by microscopy.
RESULTS
Anhydride-modified ovalbumin significantly inhibited the infection by HSV-2 with an IC(50) of 23.56±8.33 µg/ml. HP-OVA showed only low cytotoxicity to the host cells with a CC(50) over 1 mg/ml. HP-OVA did not produce significant inhibitory effect on the 17 strains of vaginal lactobacilli (MIC>1 mg/ml).
CONCLUSION
Anhydride-modified protein HP-OVA exhibits potent anti-HSV-2 activity in vitro and can be a good microbicide candidate for prevention of sexually transmitted diseases.
目的
研究3-羟基邻苯二甲酸酐修饰的卵清蛋白（HP-OVA）对单纯疱疹病毒2型（HSV-2）的体外抗病毒活性。
方法
通过化学修饰，用3-羟基邻苯二甲酸酐（HP）处理卵清蛋白（OVA）制备HP-OVA。采用MTT比色法检测HP-OVA对体外HSV-2 333病毒的抗HSV-2活性以及在非洲绿猴肾细胞（Vero细胞）中的细胞毒性。通过显微镜观察HP-OVA对17株阴道乳酸杆菌的抑制作用。
结果
酸酐修饰的卵清蛋白显著抑制HSV-2感染，IC(50)为23.56±8.33 μg/ml。HP-OVA对宿主细胞仅表现出低细胞毒性，CC(50)超过1 mg/ml。HP-OVA对17株阴道乳酸杆菌未产生显著抑制作用（MIC>1 mg/ml）。
结论
酸酐修饰的蛋白HP-OVA在体外表现出强大的抗HSV-2活性，可成为预防性传播疾病的良好杀菌剂候选物。
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