School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1235-1240. doi: 10.1099/ijs.0.031989-0. Epub 2011 Jul 15.
A halophilic archaeon, strain TBN51(T), was isolated from a marine solar saltern in Jiangsu, China. The colonies were red-pigmented and the cells were pleomorphic, motile and Gram-staining-negative. The strain was able to grow at 20-55 °C (optimum 42 °C), in the presence of 1.4-5.1 M NaCl (optimum 2.6 M), with 0-1.0 M MgCl(2) (optimum 0.05 M) and at pH 5.5-9.5 (optimum pH 7.0). Cells lysed in distilled water; the minimal NaCl concentration to prevent such lysis was 8 % (w/v). The major polar lipids of strain TBN51(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and an unidentified glycolipid. The latter lipid and a minor glycolipid also detected in the novel strain were chromatographically identical to sulfated and non-sulfated mannosyl glucosyl diether, respectively. Analysis revealed that strain TBN51(T) had three dissimilar 16S rRNA genes. Phylogenetic analysis based on the sequences of these genes indicated that the novel strain was most closely related to Halomicrobium mukohataei JCM 9738(T) (89.2-94.8 % sequence similarity) and Halomicrobium katesii DSM 19301(T) (88.8-94.8 %). In similar comparisons of rpoB' gene sequences, strain TBN51(T) also appeared most closely related to Hmc. mukohataei JCM 9738(T) (88.5 % sequence similarity) and Hmc. katesii DSM 19301(T) (88.1 %). The genomic DNA G+C content of strain TBN51(T) was 69.1 mol%. The results of DNA-DNA hybridizations indicated that strain TBN51(T) represented a novel species since it showed relatedness values of only 23 % with Hmc. mukohataei JCM 9738(T) and 21 % with Hmc. katesii DSM 19301(T). It was concluded that strain TBN51(T) represents a novel species of the genus Halomicrobium, for which the name Halomicrobium zhouii sp. nov. is proposed. The type strain is TBN51(T) ( = CGMCC 1.10457(T) = JCM 17095(T)).
一株嗜盐古菌，菌株 TBN51(T)，从中国江苏的一个海洋太阳能盐场中分离得到。菌落呈红色，细胞形态多样，运动且革兰氏染色阴性。该菌株能够在 20-55°C（最佳温度 42°C）、1.4-5.1 M NaCl（最佳 2.6 M）、0-1.0 M MgCl₂（最佳 0.05 M）和 pH5.5-9.5（最佳 pH7.0）的条件下生长。细胞在蒸馏水中溶解；防止这种溶解的最低 NaCl 浓度为 8%（w/v）。菌株 TBN51(T)的主要极性脂是磷脂酰甘油、磷酸甘油磷酸甲酯、磷脂酰甘油硫酸酯和一种未鉴定的糖脂。新型菌株中检测到的后一种脂类和一种次要糖脂在色谱上分别与硫酸化和非硫酸化甘露糖葡萄糖基二醚相同。分析表明，菌株 TBN51(T)有三个不同的 16S rRNA 基因。基于这些基因序列的系统发育分析表明，该新型菌株与 Halomicrobium mukohataei JCM 9738(T)（89.2-94.8%序列相似性）和 Halomicrobium katesii DSM 19301(T)（88.8-94.8%序列相似性）最为密切相关。在 rpoB'基因序列的类似比较中，菌株 TBN51(T)也与 Hmc. mukohataei JCM 9738(T)（88.5%序列相似性）和 Hmc. katesii DSM 19301(T)（88.1%序列相似性）最为密切相关。菌株 TBN51(T)的基因组 DNA G+C 含量为 69.1mol%。DNA-DNA 杂交试验的结果表明，由于菌株 TBN51(T)与 Hmc. mukohataei JCM 9738(T)的相关性值仅为 23%，与 Hmc. katesii DSM 19301(T)的相关性值仅为 21%，因此它代表了一个新的种。因此，建议将菌株 TBN51(T)命名为 Halomicrobium zhouii sp. nov.。模式菌株为 TBN51(T)（=CGMCC 1.10457(T)=JCM 17095(T)）。
