State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2682-2689. doi: 10.1099/ijs.0.025841-0. Epub 2010 Dec 17.
Four halophilic archaeal strains, designated TNN18(T), TBN12, TNN28(T) and TBN19, were isolated from brines sampled from two artificial marine solar salterns in eastern China. Strains TNN18(T) and TNN28(T) were isolated from the Tainan marine solar saltern, whereas strains TBN12 and TBN19 were from the Taibei marine solar saltern. Colonies of the four strains were red-pigmented and their cells were pleomorphic, motile, Gram-reaction-negative rods. Strains TNN18(T) and TBN12 were able to grow at 25-50 °C (optimum 37 °C), in 10-3 % (w/v) NaCl (optimum 15 %), with 0-1.0 M MgCl(2) (optimum 0.05 M) and at pH 5.5-9.0 (optimum pH 7.0-7.5), while strains TNN28(T) and TBN19 were able to grow at 20-50 °C (optimum 37 °C), in 15-30 % (w/v) NaCl (optimum 18-20 %), in 0.005-1.0 M MgCl(2) (optimum 0.01-0.3 M) and at pH 6.0-9.0 (optimum pH 7.0-7.5). Cells of these strains lyse in distilled water; minimal NaCl concentrations to prevent cell-lysis are 10 % (w/v) for strains TNN18(T) and TBN12 and 12 % (w/v) for strains TNN28(T) and TBN19. The major polar lipids of strains TNN18(T) and TBN12 were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and one major glycolipid (GL1), which was chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). Minor amounts of other lipids (GL0, GL2, GL3 and GL4) were also detectable. The polar lipid profiles of strains TNN28(T) and TBN19 contained PG, PGP-Me, GL1, which was chromatographically identical to S-DGD-1, and three to four minor unidentified glycolipids (GL2-GL5). Phylogenetic analyses revealed that strains TNN18(T) and TBN12 formed a distinct clade with strains of the closest related species, Haloquadratum walsbyi (91.5-91.8 % 16S rRNA gene sequence similarity) and strains TNN28(T) and TBN19 formed a distinct clade with strains of the species Halosimplex carlsbadense (89.9-93.3 % similarity) and two members of the genus Halorhabdus (92.5-93.3 % similarity). The DNA G+C contents of strains TNN18(T), TBN12, TNN28(T) and TBN19 were 61.5, 62.4, 61.9 and 61.5 mol%, respectively. DNA-DNA hybridization values between strains TNN18(T) and TBN12, and strains TNN28(T) and TBN19 were 82.9 % and 88.2 %, respectively. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the four strains represent two novel species of two new genera within the family Halobacteriaceae, for which the names Halobellus clavatus gen. nov., sp. nov. (type strain TNN18(T ) = CGMCC 1.10118(T ) = JCM 16424(T)) and Halorientalis regularis gen. nov., sp. nov. (type strain TNN28(T ) = CGMCC 1.10123(T ) = JCM 16425(T)) are proposed.
从中国东部两个人工海洋盐田中采集的卤水样本中分离到了 4 株嗜盐古菌菌株，分别命名为 TNN18(T)、TBN12、TNN28(T)和 TBN19。菌株 TNN18(T)和 TNN28(T)分离自台南海洋盐田，而菌株 TBN12 和 TBN19 则来自台北海洋盐田。这 4 株菌的菌落呈红色，细胞形态多形，运动，革兰氏反应阴性的杆状。菌株 TNN18(T)和 TBN12 能够在 25-50°C（最佳 37°C）、10-3%（w/v）NaCl（最佳 15%）、0-1.0 M MgCl2（最佳 0.05 M）和 pH 5.5-9.0（最佳 pH 7.0-7.5）的条件下生长，而菌株 TNN28(T)和 TBN19 则能够在 20-50°C（最佳 37°C）、15-30%（w/v）NaCl（最佳 18-20%）、0.005-1.0 M MgCl2（最佳 0.01-0.3 M）和 pH 6.0-9.0（最佳 pH 7.0-7.5）的条件下生长。这些菌株的细胞在蒸馏水中溶解；防止细胞溶解的最小 NaCl 浓度分别为 10%（w/v）用于菌株 TNN18(T)和 TBN12，12%（w/v）用于菌株 TNN28(T)和 TBN19。菌株 TNN18(T)和 TBN12 的主要极性脂为磷脂酰甘油（PG）、磷酸甘油磷酸甲酯（PGP-Me）、磷脂酰甘油硫酸盐（PGS）和一种主要糖脂（GL1），该糖脂在色谱上与硫酸甘露糖葡糖基醚（S-DGD-1）相同。还可以检测到少量其他脂质（GL0、GL2、GL3 和 GL4）。菌株 TNN28(T)和 TBN19 的极性脂图谱含有 PG、PGP-Me、GL1，与 S-DGD-1 在色谱上相同，以及三到四种少量未鉴定的糖脂（GL2-GL5）。系统发育分析表明，菌株 TNN18(T)和 TBN12 与最接近的种 Haloquadratum walsbyi（16S rRNA 基因序列相似度为 91.5-91.8%）形成一个独特的分支，而菌株 TNN28(T)和 TBN19 与种 Halosimplex carlsbadense（相似度为 89.9-93.3%）和两个属 Halorhabdus（相似度为 92.5-93.3%）的两个成员形成一个独特的分支。菌株 TNN18(T)、TBN12、TNN28(T)和 TBN19 的 DNA G+C 含量分别为 61.5、62.4、61.9 和 61.5 mol%。菌株 TNN18(T)和 TBN12 之间以及菌株 TNN28(T)和 TBN19 之间的 DNA-DNA 杂交值分别为 82.9%和 88.2%。表型、化学分类和系统发育特性表明，这 4 株菌代表了嗜盐杆菌科两个新属的两个新种，分别命名为 Halobellus clavatus gen. nov.，sp. nov.（模式菌株 TNN18(T) = CGMCC 1.10118(T) = JCM 16424(T)）和 Halorientalis regularis gen. nov.，sp. nov.（模式菌株 TNN28(T) = CGMCC 1.10123(T) = JCM 16425(T)）。
