Zaki S R, Austin G E, Chan W C, Conaty A L, Trusler S, Trappier S, Lindsey R B, Swan D C
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
Genes Chromosomes Cancer. 1990 Nov;2(4):266-70. doi: 10.1002/gcc.2870020403.
Oligonucleotide probes have been used to map the myeloperoxidase (MPO) gene locus to chromosome bands 17q21-22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72-base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium-labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.
寡核苷酸探针已被用于将髓过氧化物酶（MPO）基因位点定位到染色体带17q21 - 22。这与使用传统cDNA探针报道的结果一致。未获得存在第二个MPO基因位点的证据。对应于MPO基因不同外显子区域的六种合成72碱基寡核苷酸被氚标记并用作原位杂交探针。合成寡核苷酸探针为基因定位提供了一种有用的替代传统DNA探针的方法。
