Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Front Immunol. 2021 Nov 22;12:572592. doi: 10.3389/fimmu.2021.572592. eCollection 2021.
The pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.
We used label-free quantification combined with liquid chromatography mass spectrometry (LC-MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by experiments.
There were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log|FC| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.
The overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
强直性脊柱炎（AS）的发病机制尚未阐明，特别是涉及髋关节疾病。本研究旨在分析 AS 患者髋关节疾病的蛋白质组，并鉴定关键蛋白生物标志物。
我们使用无标记定量结合液相色谱-质谱联用（LC-MS/MS）技术筛选 AS 组和非 AS 组髋关节韧带样本中的差异表达蛋白。通过生物信息学方法筛选关键蛋白，并通过实验进行验证。
共鉴定出 3755 种蛋白质，其中 92.916%可定量。根据 P<0.01 和 Log|FC|>1，共鉴定出 193 个差异表达蛋白（49 个上调蛋白和 144 个下调蛋白）。差异表达蛋白主要参与细胞区室，包括液泡腔、嗜天青颗粒、初级溶酶体等。主要的 KEGG 途径包括吞噬体、甘油磷脂代谢、赖氨酸降解、戊糖磷酸途径。髓过氧化物酶（MPO）被鉴定为参与吞噬体途径的关键蛋白。siRNA 干扰细胞的实验进一步证实，上调的 MPO 可能通过吞噬体途径促进成纤维细胞的炎症反应。
MPO 的过表达可能通过吞噬体途径促进 AS 受累髋关节的自身免疫炎症反应。
