• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

以若丹明123在CD8细胞中的蓄积作为替代指标，研究盐酸千金藤素在体内对P-糖蛋白的调节作用。

Using rhodamine 123 accumulation in CD8 cells as a surrogate indicator to study the P-glycoprotein modulating effect of cepharanthine hydrochloride in vivo.

作者信息

Li Han, Yan Zhang, Ning Wang, Xiao-Juan Guo, Cai-Hong Zang, Jin-Hua Jiang, Fang Ma, Qing-Duan Wang

机构信息

School of Pharmaceutical Sciences, Zhengzhou University, Zhenzhou 45001, Henan, China.

出版信息

J Biomed Biotechnol. 2011;2011:281651. doi: 10.1155/2011/281651. Epub 2011 Jun 30.

DOI:10.1155/2011/281651
PMID:21765632
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3134191/
Abstract

The purpose of this study was the use of rhodamine 123 (Rho123) accumulation in peripheral blood CD8(+)cells as a surrogate indicator to evaluate the modulating effect of P-glycoprotein (P-gp) inhibitors in the multidrug resistance (MDR) tumor-bearing mouse model. Rho123 was administered to mice, and the fluorescence level in CD8(+) cells was measured. Cepharanthine hydrochloride (CH) and verapamil (VER), two P-gp inhibitors, were administered to mice 1 hour prior to Rho123 administration in vivo or added to peripheral blood 1 hour prior to Rho123 addition ex vivo. The tumor inhibition effect of 5-fluorouracil/adriamycin/cisplatin (FAP) protocol plus CH was also investigated. A concentration- or dose-response relationship was shown between the concentration and dose of CH and Rho123 accumulation or the antitumor activity. In conclusion, the measurement of Rho123 accumulation in CD8(+) cells provides a surrogate assay for the screening of candidate P-gp inhibitors in preclinical trials, and CH is effective in modulating P-gp-mediated MDR in vivo.

摘要

本研究的目的是利用外周血CD8(+)细胞中罗丹明123（Rho123）的蓄积作为替代指标，评估P-糖蛋白（P-gp）抑制剂在多药耐药（MDR）荷瘤小鼠模型中的调节作用。给小鼠注射Rho123，并测量CD8(+)细胞中的荧光水平。在体内给小鼠注射Rho123前1小时给予两种P-gp抑制剂盐酸千金藤素（CH）和维拉帕米（VER），或在体外添加Rho123前1小时将其添加到外周血中。还研究了5-氟尿嘧啶/阿霉素/顺铂（FAP）方案加CH的抑瘤效果。CH的浓度或剂量与Rho123蓄积或抗肿瘤活性之间呈现浓度-或剂量-反应关系。总之，测量CD8(+)细胞中Rho123的蓄积为临床前试验中筛选候选P-gp抑制剂提供了一种替代检测方法，并且CH在体内有效调节P-gp介导的MDR。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/d1d09913c67b/JBB2011-281651.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/84f119257895/JBB2011-281651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/cba042986586/JBB2011-281651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/630d1f20b177/JBB2011-281651.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/d1d09913c67b/JBB2011-281651.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/84f119257895/JBB2011-281651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/cba042986586/JBB2011-281651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/630d1f20b177/JBB2011-281651.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e6/3134191/d1d09913c67b/JBB2011-281651.004.jpg

相似文献

1
Using rhodamine 123 accumulation in CD8 cells as a surrogate indicator to study the P-glycoprotein modulating effect of cepharanthine hydrochloride in vivo.以若丹明123在CD8细胞中的蓄积作为替代指标，研究盐酸千金藤素在体内对P-糖蛋白的调节作用。
J Biomed Biotechnol. 2011;2011:281651. doi: 10.1155/2011/281651. Epub 2011 Jun 30.
2
[Correlation between reversing effect of cepharanthine hydrochloride on multidrug resistance and P-glycoprotein expression and function of K562/ADR cells].盐酸千金藤素对K562/ADR细胞多药耐药的逆转作用与P-糖蛋白表达及功能的相关性
Yao Xue Xue Bao. 2012 May;47(5):594-9.
3
Flow cytometric functional analysis of multidrug resistance by Fluo-3: a comparison with rhodamine-123.利用Fluo-3通过流式细胞术对多药耐药性进行功能分析：与罗丹明-123的比较。
Eur J Cancer. 1995 Sep;31A(10):1682-8. doi: 10.1016/0959-8049(95)00288-t.
4
Reversal of P-gp mediated multidrug resistance in-vitro and in-vivo by FG020318.FG020318在体外和体内逆转P-糖蛋白介导的多药耐药性。
J Pharm Pharmacol. 2004 Aug;56(8):1061-6. doi: 10.1211/0022357043879.
5
Reversal effect of FW-04-806, a macrolide dilactone compound, on multidrug resistance mediated by ABCB1 and ABCG2 in vitro and in vivo.FW-04-806，一种大环内酯类双内酯化合物，对 ABCB1 和 ABCG2 介导的多药耐药的体内外逆转作用。
Cell Commun Signal. 2019 Sep 1;17(1):110. doi: 10.1186/s12964-019-0408-5.
6
Overcoming multidrug-resistance in vitro and in vivo using the novel P-glycoprotein inhibitor 1416.使用新型 P 糖蛋白抑制剂 1416 克服体外和体内的多药耐药性。
Biosci Rep. 2012 Dec;32(6):559-66. doi: 10.1042/BSR20120020.
7
In vitro and in vivo multidrug resistance reversal activity by a Betti-base derivative of tylosin.Betti 碱衍生泰乐菌素的体外和体内多药耐药逆转活性。
Br J Cancer. 2010 Jul 13;103(2):178-85. doi: 10.1038/sj.bjc.6605716. Epub 2010 Jun 15.
8
A human lymphocyte based ex vivo assay to study the effect of drugs on P-glycoprotein (P-gp) function.一种基于人淋巴细胞的体外试验，用于研究药物对P-糖蛋白（P-gp）功能的影响。
Pharm Res. 2001 Jan;18(1):39-44. doi: 10.1023/a:1011070509191.
9
Tetrandrine and fangchinoline, bisbenzylisoquinoline alkaloids from Stephania tetrandra can reverse multidrug resistance by inhibiting P-glycoprotein activity in multidrug resistant human cancer cells.汉防己甲素和汉防己乙素，从粉防己中提取的双苄基异喹啉生物碱，可通过抑制多药耐药人癌细胞中的P-糖蛋白活性来逆转多药耐药性。
Phytomedicine. 2014 Jul-Aug;21(8-9):1110-9. doi: 10.1016/j.phymed.2014.04.029. Epub 2014 May 22.
10
The multidrug resistance of tumour cells was reversed by tetrandrine in vitro and in xenografts derived from human breast adenocarcinoma MCF-7/adr cells.汉防己甲素在体外以及来源于人乳腺腺癌MCF-7/adr细胞的异种移植瘤中均可逆转肿瘤细胞的多药耐药性。
Eur J Cancer. 2002 Feb;38(3):418-26. doi: 10.1016/s0959-8049(01)00356-2.

引用本文的文献

1
Viral hijacking of host DDX60 promotes Crimean-Congo haemorrhagic fever virus replication via G-quadruplex unwinding.病毒劫持宿主DDX60通过解旋G-四链体促进克里米亚-刚果出血热病毒复制。
PLoS Pathog. 2025 Jun 27;21(6):e1013278. doi: 10.1371/journal.ppat.1013278. eCollection 2025 Jun.
2
Pharmacological Effects and Clinical Prospects of Cepharanthine.盐酸千金藤碱的药理作用及临床前景。
Molecules. 2022 Dec 15;27(24):8933. doi: 10.3390/molecules27248933.
3
Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine.

本文引用的文献

1
In vitro and in vivo multidrug resistance reversal activity by a Betti-base derivative of tylosin.Betti 碱衍生泰乐菌素的体外和体内多药耐药逆转活性。
Br J Cancer. 2010 Jul 13;103(2):178-85. doi: 10.1038/sj.bjc.6605716. Epub 2010 Jun 15.
2
Future perspectives in hepatocellular carcinoma.肝细胞癌的未来展望。
Dig Liver Dis. 2010 Jul;42 Suppl 3:S302-9. doi: 10.1016/S1590-8658(10)60521-X.
3
Nanomedicinal strategies to treat multidrug-resistant tumors: current progress.纳米医学策略治疗多药耐药肿瘤：当前进展。
天然抗癌化合物：源自中草药的光芒。
Chin Med. 2019 Nov 6;14:48. doi: 10.1186/s13020-019-0270-9. eCollection 2019.
4
Proteomic Differences in Feline Fibrosarcomas Grown Using Doxorubicin-Sensitive and -Resistant Cell Lines in the Chick Embryo Model.鸡胚模型中使用阿霉素敏感和耐药细胞系生长的猫纤维肉瘤的蛋白质组差异。
Int J Mol Sci. 2018 Feb 14;19(2):576. doi: 10.3390/ijms19020576.
5
Determination of cepharanthine in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.采用液相色谱-串联质谱法测定大鼠血浆中的千金藤素及其在药代动力学研究中的应用。
Pharm Biol. 2017 Dec;55(1):1775-1779. doi: 10.1080/13880209.2017.1328446.
6
Guizhi Fuling Wan, a Traditional Chinese Herbal Formula, Sensitizes Cisplatin-Resistant Human Ovarian Cancer Cells through Inactivation of the PI3K/AKT/mTOR Pathway.桂枝茯苓丸，一种传统中药配方，通过使PI3K/AKT/mTOR信号通路失活来增敏顺铂耐药的人卵巢癌细胞。
Evid Based Complement Alternat Med. 2016;2016:4651949. doi: 10.1155/2016/4651949. Epub 2016 May 18.
7
P-Glycoprotein Activity in Steroid-Responsive vs. Steroid-Resistant Nephrotic Syndrome.类固醇反应性与类固醇抵抗性肾病综合征中的P-糖蛋白活性
Indian J Pediatr. 2016 Nov;83(11):1222-1226. doi: 10.1007/s12098-016-2142-y. Epub 2016 May 19.
8
New fluphenazine analogues as inhibitors of P-glycoprotein in human lymphocyte cultures.新型氟奋乃静类似物作为人淋巴细胞培养物中P-糖蛋白的抑制剂
Contemp Oncol (Pozn). 2012;16(4):332-7. doi: 10.5114/wo.2012.30063. Epub 2012 Sep 29.
9
Effects of Astragalus polysaccharides on P-glycoprotein efflux pump function and protein expression in H22 hepatoma cells in vitro.黄芪多糖对体外 H22 肝癌细胞 P-糖蛋白外排泵功能及蛋白表达的影响。
BMC Complement Altern Med. 2012 Jul 11;12:94. doi: 10.1186/1472-6882-12-94.
Nanomedicine (Lond). 2010 Jun;5(4):597-615. doi: 10.2217/nnm.10.35.
4
Green tea catechins augment the antitumor activity of doxorubicin in an in vivo mouse model for chemoresistant liver cancer.绿茶儿茶素增强了多柔比星在体内耐药肝癌小鼠模型中的抗肿瘤活性。
Int J Oncol. 2010 Jul;37(1):111-23.
5
Systemic treatment of hepatocellular carcinoma: dawn of a new era?肝细胞癌的系统治疗：新时代的曙光？
Ann Surg Oncol. 2010 May;17(5):1247-56. doi: 10.1245/s10434-010-0975-6. Epub 2010 Apr 20.
6
Effect of honey and eugenol on Ehrlich ascites and solid carcinoma.蜂蜜和丁香酚对艾氏腹水癌和实体癌的作用。
J Biomed Biotechnol. 2010;2010:989163. doi: 10.1155/2010/989163. Epub 2010 Mar 28.
7
[Binocular diplopia and ptosis due to snakebite (Agkistrodon blomhoffi "mamushi")--a case report].
Brain Nerve. 2010 Mar;62(3):273-7.
8
Absence of both cytochrome P450 3A and P-glycoprotein dramatically increases docetaxel oral bioavailability and risk of intestinal toxicity.细胞色素P450 3A和P-糖蛋白均缺失会显著提高多西他赛的口服生物利用度以及肠道毒性风险。
Cancer Res. 2009 Dec 1;69(23):8996-9002. doi: 10.1158/0008-5472.CAN-09-2915. Epub 2009 Nov 17.
9
A phase I study of the P-glycoprotein antagonist tariquidar in combination with vinorelbine.P-糖蛋白拮抗剂 tariquidar 联合长春瑞滨的 I 期研究。
Clin Cancer Res. 2009 May 15;15(10):3574-82. doi: 10.1158/1078-0432.CCR-08-0938. Epub 2009 May 5.
10
In vitro cytotoxicity of CD8+ T cells in multi-drug-resistant tuberculosis. A preliminary report.多药耐药结核病中CD8 + T细胞的体外细胞毒性。初步报告。
Respirology. 2009 May;14(4):574-8. doi: 10.1111/j.1440-1843.2008.01478.x. Epub 2009 Jan 23.