梗阻性黄疸对肾钠钾ATP酶活性和ATP含量的影响、它们对大肠杆菌或内毒素的反应以及1,6 - 二磷酸果糖的作用。

Effect of obstructive jaundice on renal Na-K ATPase activity and ATP contents, their response to E. coli or endotoxin and effect of 1-6 fructose diphosphate.

作者信息

Gu Y, Wang W G, Gu Y M, Gu J B, Lin S Y

机构信息

Division of Nephrology, Huashan Hospital, Shanghai Medical University.

出版信息

Chin Med J (Engl). 1990 Oct;103(10):811-6.

PMID:2176582

Abstract

A two-week bile duct ligation (BDL) in Sprague-Dawley (SD) rats raised the serum billirubin level and decreased the mean arterial blood pressure and renal cortical ATP contents compared with those in sham-operated (SO) rats (3.6 +/- 1.15 mg% vs 0.54 +/- 0.36, P less than 0.001; 69 +/- 24 mmHg vs 86 +/- 21, P less than 0.05; 3.72 +/- 0.86 x 10(-10) mol/mg tissue vs 7.27 +/- 0.18, P less than 0.05). No difference could be found in the medullary ATP contents (8.42 +/- 2.20 vs 8.70 +/- 2.80, P = NS). In SO rats, injection of endotoxin (0.7 mg/kg BW) and E. coli (3.1 x 10(5) bacteria/100 g BW) reduced cortical ATP content to 1.86 +/- 0.97 and 1.30 +/- 0.47 (P less than 0.001), and medullar ATP to 1.33 +/- 0.31 and 2.12 +/- 0.46 (P less than 0.001) respectively. In BDL rats, the same treatment led to further decrease in cortical ATP to 1.25 +/- 0.40 and 0.62 +/- 0.20, medullary ATP to 0.97 +/- 0.41 and 1.64 +/- 0.83 (P less than 0.001). Basal Na-K ATPase activity in BDL is the same compared with that in SO both in the cortex (2.85 +/- 2.2 mumol/mgpr/h vs 2.19 +/- 0.75; P = NS) and medulla (2.79 +/- 1.83 vs 3.05 +/- 1.38; P = NS).(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

与假手术（SO）大鼠相比，对Sprague-Dawley（SD）大鼠进行为期两周的胆管结扎（BDL）可使血清胆红素水平升高，平均动脉血压降低，肾皮质ATP含量减少（3.6±1.15mg% 对0.54±0.36，P<0.001；69±24mmHg对86±21，P<0.05；3.72±0.86×10⁻¹⁰mol/mg组织对7.27±0.18，P<0.05）。髓质ATP含量未发现差异（8.42±2.20对8.70±2.80，P=无显著性差异）。在SO大鼠中，注射内毒素（0.7mg/kg体重）和大肠杆菌（3.1×10⁵细菌/100g体重）可使皮质ATP含量分别降至1.86±0.97和1.30±0.47（P<0.001），髓质ATP分别降至1.33±0.31和2.12±0.46（P<0.001）。在BDL大鼠中，相同处理导致皮质ATP进一步降至1.25±0.40和0.62±0.20，髓质ATP降至0.97±0.41和1.64±0.83（P<0.001）。BDL大鼠皮质和髓质的基础钠钾ATP酶活性与SO大鼠相比无差异（皮质：2.85±2.2μmol/mg蛋白/小时对2.19±0.75；P=无显著性差异；髓质：2.79±1.83对3.05±1.38；P=无显著性差异）。（摘要截断于250字）