Li L Y, Wang H Y, Shi J H
Nephrology Institute, Beiing Medical University.
Zhonghua Yi Xue Za Zhi. 1994 Jan;74(1):9-12, 61.
To study the protective effect of 1,6-diphosphate fructose (FDP) on acute ischemia damage in brain, heart and liver, we observed its effect on acute ischemic renal failure (AIRF). AIRF in male Wistar rats was produced by renal arterial clamping for 45 minutes and reperfusion for 90 minutes. The rats were divided into 4 groups according to their age and FDP therapy: G1: young (3-4 months) AIRF rats without FDP; G2: old (26-27 months) AIRF rats without FDP; G3: young AIRF rats with FDP (0.5g/kg) i.v. infusion; G4: old AIRF rats with FDP. Inulin clearance (CIn), PAH clearance (CPAH), urinary sodium (UNA) were examined before ischemia and 30, 60 and 90 minutes after reperfusion. After reperfusion for 90 minutes, renal cortex was taken for the examination of renal mitochondrial ATP synthetic content (miATPs). CIn and CPAH were lower in G2 than in G1 (0.24 +/- 0.12 vs. 1.75 +/- 0.79, P < 0.01; 0.87 +/- 0.42 vs 7.12 +/- 4.04, P < 0.05, at 90 minutes reperfusion). CIn and CPAH in G1 showed an auto-recovery up to 40.1% and 33.7% of the level in contrast to G2. In G3 and G4 original FDP infusion resulted in a significant increase in miATPs and CIn (609 +/- 145 vs 389 +/- 97; 569 +/- 77 vs 338 +/- 61, P < 0.02; 2.47 +/- 0.58 vs 1.75 +/- 0.79, 1.29 +/- 0.42 vs 0.24 +/- 0.12, P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
为研究1,6 - 二磷酸果糖（FDP）对脑、心、肝急性缺血损伤的保护作用，我们观察了其对急性缺血性肾衰竭（AIRF）的影响。雄性Wistar大鼠的AIRF通过肾动脉夹闭45分钟及再灌注90分钟制成。根据年龄和FDP治疗情况将大鼠分为4组：G1：年轻（3 - 4个月）未用FDP的AIRF大鼠；G2：年老（26 - 27个月）未用FDP的AIRF大鼠；G3：年轻AIRF大鼠静脉输注FDP（0.5g/kg）；G4：年老AIRF大鼠用FDP。在缺血前及再灌注后30、60和90分钟检测菊粉清除率（CIn）、对氨基马尿酸清除率（CPAH）、尿钠（UNA）。再灌注90分钟后，取肾皮质检测肾线粒体ATP合成含量（miATPs）。G2组的CIn和CPAH低于G1组（再灌注90分钟时，分别为0.24±0.12对1.75±0.79，P<0.01；0.87±0.42对7.12±4.04，P<0.05）。与G2组相比，G1组的CIn和CPAH自动恢复至对照水平的40.1%和33.7%。在G3组和G4组，最初的FDP输注使miATPs和CIn显著增加（分别为609±145对389±97；569±77对338±61，P<0.02；2.47±0.58对1.75±0.79，1.29±0.42对0.24±0.12，P<0.01）。（摘要截短于250字）
