Department of Molecular Biology, Laboratory of Molecular Parasitology, W. Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Str., 00-818, Warsaw, Poland.
Parasitol Res. 2012 Jan;110(1):395-402. doi: 10.1007/s00436-011-2504-3. Epub 2011 Jul 16.
Fasciolosis is a hepatic parasitic infection that affects many mammal species and creates a great economic and veterinary problem. Molecular mechanisms of parasite-hepatocyte interactions have not been precisely characterized yet. Therefore, the aim of the study was to investigate alterations in the metabolic activity of rat liver cells exposed to Fasciola hepatica somatic proteins. Hepatocytes were incubated with 0-1 mg/ml of fluke's somatic proteins for various periods of time. Afterward, changes in hepatocytes metabolic activity were determined with MTT and enzyme leakage tests. Hepatocytes' capacity to synthesize albumin was also investigated. It was observed that protein concentration, as well as longevity of their action, influenced metabolic activity of rat liver cells. Diminution of hepatocytes survival rate, an increase in enzyme leakage and altered synthetic capacity after treatment with parasite's proteins were reported. It is concluded that somatic proteins of F. hepatica may play an important role in liver cell damaging.
肝片形吸虫病是一种肝寄生虫感染，影响许多哺乳动物物种，并造成巨大的经济和兽医问题。寄生虫-肝细胞相互作用的分子机制尚未得到精确描述。因此，本研究的目的是研究暴露于肝片形吸虫体蛋白的大鼠肝细胞代谢活性的变化。将肝细胞与 0-1mg/ml 的吸虫体蛋白孵育不同时间。之后，通过 MTT 和酶漏出试验来确定肝细胞代谢活性的变化。还研究了肝细胞合成白蛋白的能力。结果表明，蛋白质浓度以及其作用的持久性会影响大鼠肝细胞的代谢活性。据报道，用寄生虫蛋白处理后，肝细胞存活率降低，酶漏出增加，合成能力改变。结论认为，肝片形吸虫的体蛋白可能在肝细 胞损伤中起重要作用。
