Korba B E, Boumpas D, Mann D, Yoakum G H
Division of Molecular Virology and Immunology, Georgetown University Medical Center, Rockville, Maryland.
J Med Virol. 1990 Aug;31(4):272-6. doi: 10.1002/jmv.1890310406.
A transient depression of HBV serologic markers has been reported for some chronically infected patients treated with human interferons. To determine a molecular basis for these observations, a human, HBV-carrying, hepatocellular carcinoma cell line (PLC/PRF/5) was treated with human alpha, beta, or gamma interferons. Administration of these interferons resulted in a marked depression of HBV surface antigen (HG-sAg) levels in the culture medium. This inhibition was transient, with media levels of HBsAg rising substantially within 48 hours following the termination of interferon treatment. Cell growth rates were not affected by alpha interferon treatment, indicating that overall cell protein synthesis was not substantially altered. Although all three classes of interferons were effective in lowering HBsAg levels in the culture medium, intracellular levels of HBsAg-specific RNA were unaffected. These results suggest that the transient depression of HBV serologic markers in interferon-treated patients may be a consequence of the failure to disrupt the intracellular pools of HBV RNA in the liver.
据报道，一些接受人干扰素治疗的慢性感染患者出现了乙肝病毒血清学标志物的短暂下降。为了确定这些观察结果的分子基础，用人α、β或γ干扰素处理了一种携带乙肝病毒的人肝癌细胞系（PLC/PRF/5）。给予这些干扰素导致培养基中乙肝表面抗原（HBsAg）水平显著下降。这种抑制是短暂的，在干扰素治疗终止后48小时内，培养基中HBsAg水平大幅上升。细胞生长速率不受α干扰素治疗的影响，这表明总体细胞蛋白质合成没有实质性改变。虽然所有三类干扰素都能有效降低培养基中HBsAg水平，但细胞内HBsAg特异性RNA水平未受影响。这些结果表明，干扰素治疗患者中乙肝病毒血清学标志物的短暂下降可能是未能破坏肝脏中乙肝病毒RNA细胞内池的结果。
