Nakajima Y, Kuwata T, Tomita Y, Okuda K
Microbiol Immunol. 1982;26(8):705-12. doi: 10.1111/j.1348-0421.1982.tb00213.x.
The effects of human alpha and beta interferons (IFN) on the production of HBsAG by PLC/PRF/5 cells, an HBsAg-producing human hepatoma cell line, were studied in the exponential and stationary phases of cell growth. When exponential phase cells were treated with 100 or 1,000 U of IFN per ml for 48 hr. the amount of HBsAg in the culture medium decreased. The number of cells and the synthesis of DNA and proteins were also reduced by the IFN treatment. These results suggested that IFN did not affect the production of HBsAg specifically in exponential phase cells. When cells in the stationary phase were similarly treated with IFN, HbsAg production was not inhibited nor did the number of cells decrease. To examine the antiviral state induced by IFN in PLC/PRF/5, induction of 2'5'-oligo (A) synthetase and susceptibility to two kinds of viruses were examined. The 2'5'-oligo (A) synthetase activity was increased in an IFN-dose dependent manner. Susceptibility to vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV) was decreased by treatment with 10 and 100 U of IFN per ml for 20 hr. It was concluded that IFN-alpha and IFN-beta induce 2'5'-oligo (A) synthetase and the antiviral state, but do not inhibit HBsAg production by PLC/PRF/5 cells.
研究了人α和β干扰素（IFN）对PLC/PRF/5细胞（一种产生HBsAg的人肝癌细胞系）在细胞生长指数期和静止期产生HBsAg的影响。当指数期细胞用每毫升100或1000单位的IFN处理48小时时，培养基中HBsAg的量减少。IFN处理还降低了细胞数量以及DNA和蛋白质的合成。这些结果表明，IFN在指数期细胞中并非特异性地影响HBsAg的产生。当静止期细胞用IFN进行类似处理时，HBsAg的产生未受抑制，细胞数量也未减少。为了检测IFN在PLC/PRF/5中诱导的抗病毒状态，检测了2',5'-寡聚（A）合成酶的诱导情况以及对两种病毒的敏感性。2',5'-寡聚（A）合成酶活性以IFN剂量依赖性方式增加。用每毫升10和100单位的IFN处理20小时后，对水疱性口炎病毒（VSV）和脑心肌炎病毒（EMCV）的敏感性降低。得出的结论是，IFN-α和IFN-β诱导2',5'-寡聚（A）合成酶和抗病毒状态，但不抑制PLC/PRF/5细胞产生HBsAg。
