表皮生长因子对下丘脑-垂体-肾上腺轴的刺激作用。

Stimulation of the hypothalamic-pituitary-adrenal axis by epidermal growth factor.

作者信息

Miyabo S, Ooya E, Miyanaga K, Aoyagi N, Hirai M, Kishida S, Nakai T

机构信息

Department of Internal Medicine, Fukui Medical School, Japan.

出版信息

Regul Pept. 1990 Oct 29;31(1):65-74. doi: 10.1016/0167-0115(90)90196-4.

DOI:10.1016/0167-0115(90)90196-4

PMID:2176727

Abstract

The effects of mouse epidermal growth factor (mEGF) on the hypothalamic-pituitary-adrenocortical axis were studied in vivo in conscious male rats and in vitro with cultured anterior pituitary cells. Both intravenous (i.v.) and intracerebroventricular (i.c.v.) injections of mEGF (5-20 ng: 8.3-33.3 pmol) produced significant, dose-related increases in plasma ACTH and corticosterone concentrations. The potency of mEGF is 1/20-1/50 of that of rat corticotropin-releasing factor (rCRF), and pretreatment with 150 micrograms alpha-helical CRF (9-41) completely abolished the effects of the two peptides. mEGF in concentrations ranging from 10 pM to 10 nM did not significantly affect ACTH release from dispersed anterior pituitary cells. It also failed to alter ACTH secretion in response to rCRF. These results indicate that mEGF stimulates the pituitary-adrenocortical axis through a CRF-dependent mechanism.

摘要

在清醒雄性大鼠体内以及利用培养的垂体前叶细胞进行体外实验，研究了小鼠表皮生长因子（mEGF）对下丘脑 - 垂体 - 肾上腺皮质轴的影响。静脉内（i.v.）和脑室内（i.c.v.）注射mEGF（5 - 20 ng：8.3 - 33.3 pmol）均能使血浆促肾上腺皮质激素（ACTH）和皮质酮浓度显著升高，且呈剂量依赖性。mEGF的效力为大鼠促肾上腺皮质激素释放因子（rCRF）的1/20 - 1/50，用150微克α - 螺旋CRF（9 - 41）预处理可完全消除这两种肽的作用。浓度范围为10 pM至10 nM的mEGF对分散的垂体前叶细胞释放ACTH无显著影响，也未能改变其对rCRF的ACTH分泌反应。这些结果表明，mEGF通过一种依赖CRF的机制刺激垂体 - 肾上腺皮质轴。