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釉基质衍生物和转化生长因子-β1 对人成骨细胞的影响。

Effects of enamel matrix derivative and transforming growth factor-β1 on human osteoblastic cells.

机构信息

Department of Oral Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av, do Café s/n, 14040-904 Ribeirão Preto, SP, Brazil.

出版信息

Head Face Med. 2011 Jul 18;7:13. doi: 10.1186/1746-160X-7-13.

DOI:10.1186/1746-160X-7-13
PMID:21767401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3162535/
Abstract

BACKGROUND

Extracellular matrix proteins are key factors that influence the regenerative capacity of tissues. The objective of the present study was to evaluate the effects of enamel matrix derivative (EMD), TGF-β1, and the combination of both factors (EMD+TGF-β1) on human osteoblastic cell cultures.

METHODS

Cells were obtained from alveolar bone of three adult patients using enzymatic digestion. Effects of EMD, TGF-β1, or a combination of both were analyzed on cell proliferation, bone sialoprotein (BSP), osteopontin (OPN) and alkaline phosphatase (ALP) immunodetection, total protein synthesis, ALP activity and bone-like nodule formation.

RESULTS

All treatments significantly increased cell proliferation compared to the control group at 24 h and 4 days. At day 7, EMD group showed higher cell proliferation compared to TGF-β1, EMD + TGF-β1 and the control group. OPN was detected in the majority of the cells for all groups, whereas fluorescence intensities for ALP labeling were greater in the control than in treated groups; BSP was not detected in all groups. All treatments decreased ALP levels at 7 and 14 days and bone-like nodule formation at 21 days compared to the control group.

CONCLUSIONS

The exposure of human osteoblastic cells to EMD, TGF-β1 and the combination of factors in vitro supports the development of a less differentiated phenotype, with enhanced proliferative activity and total cell number, and reduced ALP activity levels and matrix mineralization.

摘要

背景

细胞外基质蛋白是影响组织再生能力的关键因素。本研究旨在评估釉基质衍生蛋白（EMD）、TGF-β1 以及两者联合（EMD+TGF-β1）对人成骨细胞培养的影响。

方法

采用酶消化法从 3 名成年患者的牙槽骨中获取细胞。分析 EMD、TGF-β1 或两者联合对细胞增殖、骨涎蛋白（BSP）、骨桥蛋白（OPN）和碱性磷酸酶（ALP）免疫检测、总蛋白合成、ALP 活性和类骨质结节形成的影响。

结果

与对照组相比，所有治疗组在 24 小时和 4 天时均显著增加细胞增殖。在第 7 天，EMD 组的细胞增殖高于 TGF-β1 组、EMD+TGF-β1 组和对照组。所有组均检测到大多数细胞中的 OPN，而 ALP 标记的荧光强度在对照组中大于处理组；所有组均未检测到 BSP。与对照组相比，所有治疗组在第 7 天和第 14 天降低了 ALP 水平，在第 21 天降低了类骨质结节形成。

结论

体外暴露于人成骨细胞的 EMD、TGF-β1 以及两者联合因素支持发展出分化程度较低的表型，具有增强的增殖活性和细胞总数，降低 ALP 活性水平和基质矿化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/430df77a8eff/1746-160X-7-13-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/77e90649def8/1746-160X-7-13-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/52aa4a0868d9/1746-160X-7-13-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/92b3f2a741e3/1746-160X-7-13-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/4fbfe1ab3b83/1746-160X-7-13-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/dc734c7b6dd5/1746-160X-7-13-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/a3215341a448/1746-160X-7-13-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/430df77a8eff/1746-160X-7-13-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/77e90649def8/1746-160X-7-13-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/52aa4a0868d9/1746-160X-7-13-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/92b3f2a741e3/1746-160X-7-13-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/4fbfe1ab3b83/1746-160X-7-13-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/dc734c7b6dd5/1746-160X-7-13-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/a3215341a448/1746-160X-7-13-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c233/3162535/430df77a8eff/1746-160X-7-13-7.jpg

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