Department of Morphology, Institute of Biology Sciences, Federal University of Goiás, Goiás 74001-970, Brazil.
Gen Comp Endocrinol. 2011 Sep 15;173(3):419-27. doi: 10.1016/j.ygcen.2011.06.017. Epub 2011 Jul 13.
The gerbil female prostate undergoes morphological and physiological changes resulting from hormonal fluctuations that occur during the reproductive cycle. These repetitive cycles of glandular growth and regression are followed by an extensive reconstruction and remodeling of prostate stroma throughout the reproductive life of the female gerbil. The objective of this study was to evaluate the effect that the hormonal fluctuations of the reproductive cycle have on the stromal remodeling and the expression and activity of matrix metalloproteinases MMP-2 and -9 in the adult female gerbil prostate. For this, serological, ultrastructural, immunohistochemical and biochemical methods were employed. The results showed that the major stromal alteration coincide with the peak of estradiol, which occurs in estrus, and with the peak of progesterone, occurring during diestrus II. MMP-2 and -9 presented a similar pattern of expression and activity during estrous cycle. The estrus was the phase of greater expression and activity of MMP-2 and -9. On the other hand, in DI and DII, the tissue expression and activity of MMP-2 and -9 was very weak. These results are important since they suggest the involvement of estradiol and progesterone in regulating the expression and activity of MMP-2 and -9 in adult gerbil female prostate.
沙鼠的雌性前列腺会经历形态和生理上的变化，这是由生殖周期中激素波动引起的。在雌性沙鼠的整个生殖寿命中，这些腺体生长和退化的重复周期之后是前列腺基质的广泛重建和重塑。本研究的目的是评估生殖周期的激素波动对成年雌性沙鼠前列腺基质重塑以及基质金属蛋白酶 MMP-2 和 MMP-9 的表达和活性的影响。为此，采用了血清学、超微结构、免疫组织化学和生化方法。结果表明，主要的基质改变与发情期雌激素峰值和动情间期孕激素峰值同时发生。MMP-2 和 MMP-9 在发情周期中表现出相似的表达和活性模式。发情期是 MMP-2 和 MMP-9 表达和活性较高的阶段。另一方面，在 DI 和 DII 期间，MMP-2 和 MMP-9 的组织表达和活性非常弱。这些结果很重要，因为它们表明雌二醇和孕酮参与调节成年沙鼠雌性前列腺中 MMP-2 和 MMP-9 的表达和活性。
