Logan A, McDermott E E, Logan S D
Department of Physiology, Medical School, University of Birmingham, U.K.
Cell Signal. 1990;2(1):77-84. doi: 10.1016/0898-6568(90)90035-9.
When quiescent 3T3 fibroblast cells were pre-labelled with [3H]inositol and stimulated with basic fibroblast growth factor there was a stimulation of the hydrolysis of membrane lipids and the rapid production of [3H]inositol polyphosphates. Rapid and transient peaks of isomers of inositol phosphates with the chromatographic properties of inositol trisphosphates and inositol tetrakisphosphates were detectable by anion-exchange HPLC between 5 and 10 s after stimulation. These data suggest that upon stimulation the receptor for fibroblast growth factor is coupled to a phosphoinositidase C and that one of its signal-transducing pathways involves hydrolysis of inositol lipids and the production of inositol polyphosphates, some of which may act as intracellular signals mediating the cellular response. Chronic stimulation with basic fibroblast growth factor is associated with desensitization of the inositol lipid signaling pathway.
当静止的3T3成纤维细胞用[3H]肌醇预标记并用碱性成纤维细胞生长因子刺激时，膜脂水解受到刺激，[3H]肌醇多磷酸迅速产生。刺激后5至10秒内，通过阴离子交换高效液相色谱法可检测到具有肌醇三磷酸和肌醇四磷酸色谱特性的肌醇磷酸异构体的快速和瞬时峰。这些数据表明，在刺激时，成纤维细胞生长因子受体与磷酸肌醇酶C偶联，其信号转导途径之一涉及肌醇脂的水解和肌醇多磷酸的产生，其中一些可能作为介导细胞反应的细胞内信号。用碱性成纤维细胞生长因子进行慢性刺激与肌醇脂信号通路的脱敏有关。
