Aggarwal P, Saxena S
Department of Oral Pathology and Microbiology, Subharti Dental College, Meerut, India.
Indian J Cancer. 2011 Apr-Jun;48(2):211-5. doi: 10.4103/0019-509X.82897.
The present study was undertaken to detect and compare the pattern of collagen fibers in odontogenic cysts and also to find out if this methodology could be used to predict the aggressive nature of odontogenic cysts by comparing with the odontogenic tumors.
The collagen in the wall of 11 odontogenic keratocysts, 14 dentigerous cysts and 14 radicular cysts was studied histochemically by staining sections with picrosirius red and examining under polarizing microscope. This was compared to 10 cases of odontogenic tumors using Z test of proportion at 1% and 5%.
In dentigerous cysts, odontogenic keratocysts and odontogenic tumors, the predominant color of collagen fibers birefringence was found to be orangish red, whereas in radicular cysts the collagen fiber was of green color.
Similar birefringence pattern of collagen fibers between dentigerous cysts, odontogenic keratocysts and odontogenic tumors may indicate that these lesions have a common histogenesis with a broad spectrum of biological behavior and belong to the same group, i.e., are developmental in origin. Different patterns of radicular cysts suggest different biological behavior and a positive role of inflammation on polarization color of collagen fibers.
开展本研究以检测和比较牙源性囊肿中胶原纤维的模式，并通过与牙源性肿瘤进行比较，找出该方法是否可用于预测牙源性囊肿的侵袭性。
通过用苦味酸天狼星红对切片进行染色并在偏光显微镜下观察，对11例牙源性角化囊肿、14例含牙囊肿和14例根尖囊肿壁中的胶原进行组织化学研究。使用1%和5%的比例Z检验将其与10例牙源性肿瘤进行比较。
在含牙囊肿、牙源性角化囊肿和牙源性肿瘤中，发现胶原纤维双折射的主要颜色为橙红色，而在根尖囊肿中胶原纤维为绿色。
含牙囊肿、牙源性角化囊肿和牙源性肿瘤之间胶原纤维的双折射模式相似，这可能表明这些病变具有共同的组织发生学，具有广泛的生物学行为，属于同一组，即起源于发育。根尖囊肿的不同模式表明其生物学行为不同，且炎症对胶原纤维偏振颜色有积极作用。
