使用偏振显微镜下的天狼星红染色和CD44v6免疫反应性对成釉细胞瘤和口腔鳞状细胞癌中胶原蛋白的比较评估
A Comparative Evaluation of Collagen in Ameloblastoma and Oral Squamous Cell Carcinoma using Picrosirius Red Staining with Polarizing Microscopy and CD44v6 Immunoreactivity.
作者信息
Thete Sanjay G, Deshmukh Atul, Yadav Renu, Umbare Dhirajkumar, Shinde Kanchan N, Kandalkar Sachin
机构信息
Department of Oral Pathology and Microbiology Rural Dental College, Pravara Institute of Medical Sciences (DU), Loni, Maharashtra, India.
Center for Interdisciplinary Research, D Y Patil University, Navi Mumbai, Maharashtra, India.
出版信息
J Pharm Bioallied Sci. 2023 Jul;15(Suppl 2):S952-S955. doi: 10.4103/jpbs.jpbs_269_23. Epub 2023 Jul 11.
BACKGROUND
Solid multicystic ameloblastoma (SMA) is a locally aggressive, benign odontogenic tumor of odontogenic origin with greater rate of recurrence. Epithelial-mesenchymal interaction plays an important role in tooth morphogenesis that shows complete differentiation of epithelial and ectomesenchymal components to the level of tooth formation. Tumor stroma in ameloblastoma is normal mature collagen that prevents differentiation to the level of tooth formation. Current study evaluates the role of stromal elements in aggressive behavior of SMA using picrosirius red staining with polarizing microscopy and CD44v6 immunohistochemistry (IHC).
OBJECTIVES
To compare nature of collagen using picrosirius red staining under polarized microscope and IHC expression of CD44v6 marker in SMA and oral squamous cell carcinoma (OSCC).
METHODS
Thirty blocks were retrieved from departmental archives and subjected to picrosirius red staining and CD44v6 IHC staining. Slides stained with picrosirius red were observed under polarized microscope to report the birefringence pattern. IHC slides were annotated for intensity of staining of tumor cells.
RESULTS
In contrast to OSCC's 40% red, 40% yellowish-red, and 20% greenish-yellow birefringence, SMA displayed 87% red, 13% yellowish-red, and 0% greenish-yellow. Compared to OSCC, which had tumor cells stained 9% strongly, 64% moderately, 27% mildly, and 0% negatively, SMA revealed 0% strong, 10% moderate, 60% weak, and 30% negative staining.
CONCLUSION
As opposed to OSCC, which exhibited a greater quantity of greenish-yellow birefringence of immature collagen, SMA showed predominantly red birefringence, which is suggestive of mature collagen with a lack of metastasis. Comparing SMA to OSCC, the lack of significant CD44v6 positivity suggests that there has not been perineural invasion or regional metastases in SMA.
背景
实性多囊性成釉细胞瘤（SMA）是一种具有较高复发率的、起源于牙源性的局部侵袭性良性牙源性肿瘤。上皮-间充质相互作用在牙齿形态发生中起重要作用，表现为上皮和外间充质成分完全分化至牙齿形成水平。成釉细胞瘤中的肿瘤基质是正常成熟的胶原蛋白，可阻止其分化至牙齿形成水平。本研究使用偏振显微镜下的天狼星红染色和CD44v6免疫组织化学（IHC）评估基质成分在SMA侵袭性行为中的作用。
目的
通过偏振显微镜下的天狼星红染色比较SMA和口腔鳞状细胞癌（OSCC）中胶原蛋白的性质以及CD44v6标志物的IHC表达。
方法
从科室档案中检索出30个组织块，进行天狼星红染色和CD44v6 IHC染色。在偏振显微镜下观察用天狼星红染色的玻片，报告双折射模式。对IHC玻片标注肿瘤细胞的染色强度。
结果
与OSCC的40%红色、40%黄红色和20%绿黄色双折射相比，SMA显示87%红色、13%黄红色和0%绿黄色。与OSCC相比，其肿瘤细胞染色为9%强阳性、64%中等阳性、27%弱阳性和0%阴性，SMA显示0%强阳性、10%中等阳性、60%弱阳性和30%阴性染色。
结论
与表现出大量未成熟胶原蛋白绿黄色双折射的OSCC不同，SMA主要表现为红色双折射，提示成熟胶原蛋白且无转移。将SMA与OSCC比较，缺乏显著的CD44v6阳性表明SMA中没有神经周围浸润或区域转移。
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