Sezer Atakan, Usta Ufuk, Kocak Zafer, Yagci Mehmet Ali
Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.
J Cancer Res Ther. 2011 Apr-Jun;7(2):152-6. doi: 10.4103/0973-1482.82927.
To explore the protective effect of a flavonoid fractions diosmin + hesperidin (Daflon), against radiation-induced acute proctitis in an experimental rat model.
Thirty four rats were divided into four groups. The rats in Group 1 received Daflon and underwent irradiation. The rats in Group 2 received no Daflon and underwent irradiation. The rats in Group 3 received Daflon and underwent sham irradiation. The rats in Group 4 received no Daflon and underwent sham irradiation. Daflon emulsion (100 mg/kg/day) was administered via an orogastric feeding tube to the rats in groups 1 and 3 starting from 1 day prior to irradiation until the euthanasia day (day 15 following irradiation). Radiation therapy was delivered on a cobalt-60 unit using a single fraction of 17.5 Gy defined at a depth of 1 cm through an anterior portal. Slides were examined by the same pathologist under a light microscope two times in a blinded manner.
When compared to group 2, the rats of Group 1 showed less glandular distortion and less mucosal inflammation with less infiltration of the crypt epithelia by the inflammatory cells (P < 0.001). A statistically significant increase in all parameters but muscular wall thickness was observed for the rats in Group 2 as compared to the group 3 and 4.
Administration of a dose of 100 mg/kg/day of the diosmin + hesperidin resulted in decreased morphologic inflammatory changes. This drug may have protective effects against radiation-induced acute proctitis.
探讨类黄酮成分地奥司明+橙皮苷（达弗隆）对实验性大鼠模型辐射诱导的急性直肠炎的保护作用。
34只大鼠分为四组。第1组大鼠接受达弗隆并接受照射。第2组大鼠不接受达弗隆并接受照射。第3组大鼠接受达弗隆并接受假照射。第4组大鼠不接受达弗隆并接受假照射。从照射前1天开始，通过经口胃饲管向第1组和第3组大鼠给予达弗隆乳剂（100mg/kg/天），直至安乐死日（照射后第15天）。使用钴-60装置进行放射治疗，通过前野在1cm深度处给予单次17.5Gy的剂量。同一位病理学家以盲法在光学显微镜下对玻片进行两次检查。
与第2组相比，第1组大鼠的腺体变形较少，黏膜炎症较轻，隐窝上皮的炎性细胞浸润较少（P<0.001）。与第3组和第4组相比，第2组大鼠除肌壁厚度外的所有参数均有统计学显著增加。
给予100mg/kg/天的地奥司明+橙皮苷剂量可减少形态学上的炎症变化。这种药物可能对辐射诱导的急性直肠炎有保护作用。
