用于高灵敏度核酸检测的生物发光茎环探针。
Bioluminescent stem-loop probes for highly sensitive nucleic acid detection.
机构信息
Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
出版信息
Chem Commun (Camb). 2011 Sep 7;47(33):9393-5. doi: 10.1039/c1cc13495g. Epub 2011 Jul 19.
PMID:21769413
Abstract
Here, we report the first bioluminescent stem-loop probe, which is 50 times more sensitive and able to achieve a LOD 25 times lower than fluorescent stem-loop probes. Chemical generation of a signal from Renilla luciferase reduces background noise for improved quantitative utility in nucleic acid biomarker detection.
摘要
在这里，我们报告了第一个生物发光茎环探针，它的灵敏度比荧光茎环探针高 50 倍，能够达到 25 倍的更低检测限。从海肾荧光素酶中化学产生信号降低了背景噪声，提高了核酸生物标志物检测的定量实用性。
Zotero 插件