基于生物发光蛋白-DNA 杂交体的 BRET 信标进行核酸检测。
Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.
作者信息
Engelen Wouter, van de Wiel Kayleigh M, Meijer Lenny H H, Saha Bedabrata, Merkx Maarten
机构信息
Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands.
出版信息
Chem Commun (Camb). 2017 Mar 2;53(19):2862-2865. doi: 10.1039/c6cc10032e.
Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.
生物发光分子信标是采用模块化设计方法开发的，该方法依赖于明亮的纳米荧光素酶NanoLuc与Cy3受体之间的生物发光共振能量转移（BRET）。虽然传统分子信标受到背景荧光和散射的阻碍，但这些BRET信标能够直接在复杂介质中检测低至皮摩尔浓度的核酸。
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