Centre of Materials, Surfaces and Structural Systems, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Ann Biomed Eng. 2011 Oct;39(10):2510-20. doi: 10.1007/s10439-011-0350-1. Epub 2011 Jul 16.
Electrospinning was used in innovative electrospinning rigs to obtain tubular and flat fibrous structures with controlled fiber orientation with the aim to be used as scaffolds for biomedical applications, more specifically in the tissue engineering of vascular and orthopedic grafts. Gelatine and hydroxyapatite (HA)-gelatine solutions of various compositions were tried and electrospinning of continuous fibers was maintained for gelatine and up to 0.30 g/g HA-gelatine solutions in 2,2,2-trifluoroethanol (TFE). Small diameter tubular scaffolds were electrospun with axial fiber orientation and flat scaffolds were cut from fiber mats electrospun around a wired drum substrate. The fibrous mats were crosslinked using a glutaraldehyde solution and subjected to image analysis of SEM micrographs, water swelling tests, and mechanical testing. Fiber diameter in the electrospun scaffolds could be varied depending on the feed solution concentration and composition whereas fiber orientation was affected by the processing conditions. After crosslinking, the 0.30 g/g HA-gelatine scaffolds absorbed the minimum amount of water after 48 h soaking and they had the highest Young's modulus, 60 MPa, and highest strength, 3.9 MPa.
静电纺丝技术被应用于创新型静电纺丝装置中，以获得具有受控纤维取向的管状和平坦纤维结构，旨在用作生物医学应用的支架，特别是在血管和骨科移植物的组织工程中。尝试了不同组成的明胶和羟基磷灰石（HA）-明胶溶液，并在 2,2,2-三氟乙醇（TFE）中保持了明胶和高达 0.30 g/g HA-明胶溶液的连续纤维的静电纺丝。使用轴向纤维取向静电纺制了小直径管状支架，并从围绕有线鼓基底静电纺制的纤维垫上切割出扁平支架。使用戊二醛溶液对纤维垫进行交联，并对 SEM 显微照片进行图像分析、水膨胀试验和力学试验。静电纺丝支架中的纤维直径可以根据进料溶液的浓度和组成而变化，而纤维取向则受加工条件的影响。交联后，0.30 g/g HA-明胶支架在浸泡 48 小时后吸收的水分最少，杨氏模量最高为 60 MPa，强度最高为 3.9 MPa。
