Karmi Abeer, Husseini Ghaleb A, Faroun Maryam, Sowwan Mukhles
The Nanotechnology Research Laboratory, Materials Engineering Department, Al-Quds University, East Jerusalem, United Arab Emirates.
J Nanosci Nanotechnol. 2011 Jun;11(6):4675-83. doi: 10.1166/jnn.2011.4156.
To facilitate the administration of combined 5-Fluorouracil (5-FU) and gold nanoparticles (for photothermal treatment purposes), we developed 5-FU-gold-poly(lactide-co-glycolic acid) (5-FU-Au-PLGA) nanovehicles, via the nanoprecipitation method. The gold nanoparticles were incorporated inside the 5-FU-PLGA carriers using a roller mixer. Morphological analysis using atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), indicated uniform, singly separated spherical nanoparticles (NPs). Drug content, recovery and entrapment in the NPs were approximated using UV-spectrophotometer data. Approximately 26% of nanoparticles were recovered after drying. The percentage of total drug content was about 30%, and the percentage of drug entrapment reached 57%. Electrostatic Force Microscopy images confirmed the presence of gold inside the drug-loaded nanoparticles. We speculate that the 20-nm gold particles were able to diffuse, after 12 hours of mixing (using the roller mixer), into the PLGA matrix through the 100-nm pores (observed by SEM) without affecting the integrity of the drug delivery vehicle. These synthesized nanoparticles show promise as multimodal vehicles in the delivery of chemotherapeutic agents.
为便于联合使用5-氟尿嘧啶（5-FU）和金纳米颗粒（用于光热治疗），我们通过纳米沉淀法制备了5-氟尿嘧啶-金-聚乳酸-羟基乙酸共聚物（5-FU-Au-PLGA）纳米载体。使用滚筒混合器将金纳米颗粒掺入5-FU-PLGA载体中。通过原子力显微镜（AFM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）进行的形态学分析表明，纳米颗粒呈均匀、单个分离的球形。利用紫外分光光度计数据估算了纳米颗粒中的药物含量、回收率和包封率。干燥后约26%的纳米颗粒被回收。总药物含量百分比约为30%，药物包封率达到57%。静电力显微镜图像证实了载药纳米颗粒内部存在金。我们推测，在混合12小时（使用滚筒混合器）后直径20 nm的金颗粒能够通过SEM观察到的100 nm孔隙扩散到PLGA基质中，而不影响药物递送载体的完整性。这些合成的纳米颗粒有望成为化疗药物递送的多模态载体。
