Li Donghong, Diao Junlin, Zhang Jiaotao, Liu Jianchang
State Key Laboratory of Trauma, Burn and Combined Injury, 2nd Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
J Nanosci Nanotechnol. 2011 Jun;11(6):4733-8. doi: 10.1166/jnn.2011.4179.
The purpose of this research was to investigate chitosan-based composite sponge containing silver nanoparticles (Ag NPs) for wound dressing application. The composite sponge was prepared by a freeze-drying technique, and then immersed in AgNO3 solution with different concentration and autoclaved at 15 psi, 121 degrees C for 15 min for the formation of Ag NPs. The composite sponge containing Ag NPs was characterized by UV-vis spectra, XRD and SEM. The characteristic peaks of Ag in the UV-vis spectra and the XRD pattern revealed the formation of Ag-NPs. The SEM image showed that the silver particles homogeneously distributed on the surface of the composite sponge with an average particle diameter of about 60-80 nm. The contents of silver determined by ICP Single-channel scanning spectrometer were 0.032, 0.096 and 0.166 weight percentage, respectively, when the composite sponge was correspondingly treated with AgNO3 at concentrations of 0.1, 0.25 and 0.5 mM. The results of enzymic degradation in vitro indicated that the Ag-NPs could obviously promote the degradation of the composite sponge. The bacteriostatic and bactericidal properties of the new sponge were preliminarily studied in vitro by using S. aureus E. coli and P. aeruginosa as test microorganisms. The test results demonstrated that the new Ag NPs-loaded chitosan-based composite sponge possessed not only bacteriostatic, but also bactericidal activity against these test bacteria.
本研究的目的是研究含银纳米颗粒（Ag NPs）的壳聚糖基复合海绵在伤口敷料中的应用。通过冷冻干燥技术制备复合海绵，然后将其浸入不同浓度的AgNO₃溶液中，并在15磅力/平方英寸、121℃下高压灭菌15分钟以形成Ag NPs。采用紫外可见光谱、X射线衍射（XRD）和扫描电子显微镜（SEM）对含Ag NPs的复合海绵进行表征。紫外可见光谱和XRD图谱中Ag的特征峰表明形成了Ag NPs。SEM图像显示银颗粒均匀分布在复合海绵表面，平均粒径约为60 - 80 nm。当复合海绵分别用浓度为0.1、0.25和0.5 mM的AgNO₃处理时，用ICP单通道扫描光谱仪测定的银含量分别为0.032、0.096和0.166重量百分比。体外酶降解结果表明，Ag NPs能明显促进复合海绵的降解。以金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌为测试微生物，对新型海绵的抑菌和杀菌性能进行了体外初步研究。测试结果表明，新型负载Ag NPs的壳聚糖基复合海绵不仅对这些测试细菌具有抑菌活性，还具有杀菌活性。
