College of Textile and Garments, Southwest University , Chongqing 400715, China.
Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile , Chongqing 400715, China.
Biomacromolecules. 2017 Nov 13;18(11):3766-3775. doi: 10.1021/acs.biomac.7b01180. Epub 2017 Oct 11.
Silver inlaid with gold nanoparticles (Au-Ag NPs) prepared by using egg white with an average sized of 10 nm and homogeneous dispersion were tested and presented red fluorescence. Au-Ag NPs were loaded into chitosan as wound dressing (CS-Au-Ag). CS-Au-Ag released silver ions faster, in higher amount, and in a more durable manner than chitosan dressing loaded with silver nanoparticles with the same silver content (CS-Ag), consequently, showing enhanced antibacterial activity. Cytotoxicity tests indicated that CS-Au-Ag showed low cytotoxicity to L929 cells similar to CS-Ag. These data suggest that cytotoxicity, which restricts further application of silver NPs, can be eliminated by decreasing the silver content. CS-Au-Ag presented rich and well-distributed pores, good mechanical properties, and enhanced swelling and retention properties, contributing to keeping the wound moist in the presence of residual egg white. Altogether, our results suggest that CS-Au-Ag greatly promoted wound healing compared to CS-Ag in vivo, demonstrating that CS-Au-Ag presents great potential for wound dressing, promoting wound healing.
用平均粒径为 10nm 且均匀分散的蛋清制备的金纳米粒子（Au-Ag NPs）具有红色荧光。将 Au-Ag NPs 负载到壳聚糖中作为伤口敷料（CS-Au-Ag）。与负载相同银含量的纳米银的壳聚糖敷料（CS-Ag）相比，CS-Au-Ag 更快、更多、更持久地释放银离子，因此具有增强的抗菌活性。细胞毒性试验表明，CS-Au-Ag 对 L929 细胞的细胞毒性与 CS-Ag 相似，较低。这些数据表明，可通过降低银含量消除限制银 NPs 进一步应用的细胞毒性。CS-Au-Ag 呈现丰富且分布均匀的孔、良好的机械性能以及增强的溶胀和保持性能，有助于在残留蛋清的存在下保持伤口湿润。总的来说，我们的结果表明，CS-Au-Ag 与 CS-Ag 相比，体内极大地促进了伤口愈合，表明 CS-Au-Ag 具有很大的伤口敷料潜力，可促进伤口愈合。
