肺炎克雷伯菌pqq基因的克隆及大肠杆菌中吡咯喹啉醌（PQQ）的生物合成
Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli.
作者信息
Meulenberg J J, Sellink E, Loenen W A, Riegman N H, van Kleef M, Postma P W
机构信息
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
出版信息
FEMS Microbiol Lett. 1990 Sep 15;59(3):337-43. doi: 10.1016/0378-1097(90)90244-k.
We have cloned genes from Klebsiella pneumoniae which are required for pyrroloquinoline quinone (PQQ) biosynthesis. The cloned 6.7 kb fragment can complement several chromosomal pqq mutants. Escherichia coli strains are unable to synthesize PQQ but E. coli strains containing the cloned 6.7 kb K. pneumoniae fragment can synthesize PQQ in large amounts and E. coli pts mutants can be complemented on minimal glucose medium by this clone.
我们已经从肺炎克雷伯菌中克隆出了参与吡咯喹啉醌（PQQ）生物合成所需的基因。克隆得到的6.7 kb片段能够互补多个染色体pqq突变体。大肠杆菌菌株无法合成PQQ，但含有克隆的6.7 kb肺炎克雷伯菌片段的大肠杆菌菌株能够大量合成PQQ，并且该克隆能够在最低限度的葡萄糖培养基上互补大肠杆菌pts突变体。
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