通过克隆的大肠杆菌染色体DNA对嗜有机甲基杆菌中参与吡咯喹啉醌生物合成基因pqqE和pqqF的突变体进行互补。
Complementation of Methylobacterium organophilum mutants affected in pyrroloquinoline quinone biosynthesis genes pqqE and pqqF by cloned Escherichia coli chromosomal DNA.
作者信息
Turlin E, Biville F, Gasser F
机构信息
Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France.
出版信息
FEMS Microbiol Lett. 1991 Sep 15;67(1):59-63. doi: 10.1016/0378-1097(91)90444-f.
The hybrid plasmid pBGT3, a derivative of pLA2917 containing a 7.8-kb fragment of Escherichia coli DNA, was found to complement pqqE and pqqF mutants of Methylobacterium organophilum, both impaired in PQQ biosynthesis. The cloned fragment of E. coli DNA did not hybridize with DNA fragments containing pqqE or pqqF previously cloned from M. organophilum. Yet, in M. organophilum mutants, expression of pqqE and pqqF genes from E. coli resulted in a PQQ production estimated at 9-16% of the production observed in M. organophilum wild-type. The growth rate in methanol medium of the complemented M. organophilum mutants was about 60% of that of the wild-type.
杂种质粒pBGT3是pLA2917的衍生物，含有一段7.8 kb的大肠杆菌DNA片段，它能够互补嗜有机甲基杆菌中PQQ生物合成受损的pqqE和pqqF突变体。克隆的大肠杆菌DNA片段与先前从嗜有机甲基杆菌中克隆的含有pqqE或pqqF的DNA片段不杂交。然而，在嗜有机甲基杆菌突变体中，来自大肠杆菌的pqqE和pqqF基因的表达导致PQQ产量估计为嗜有机甲基杆菌野生型中观察到的产量的9%-16%。互补的嗜有机甲基杆菌突变体在甲醇培养基中的生长速率约为野生型的60%。
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