Laboratório de Enzimologia Industrial, Universidade de São Paulo, Ribeirão Preto-SP, Brazil.
Can J Microbiol. 2011 Jul;57(7):611-6. doi: 10.1139/w11-034. Epub 2011 Jul 19.
In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (6-7 h) in either the wild type or the plcA-deficient mutant. Only after 8 h of cultivation on glucose did the mutant strain present some decrease in nuclear duplication. However, decreased nuclear division rates were observed in the wild type when cultivated in media amended with polypectate, whereas our plcA-deficient mutant did not show slow nuclear duplication rates when grown on this carbon source, even though it requires induction and secretion of multiple pectinolytic enzymes to be metabolized. Thus, plcA appears to be directly linked to high-molecular-weight carbon source sensing.
在这项工作中，我们破坏了构巢曲霉中三个假定的磷脂酰肌醇磷脂酶 C 基因之一，并研究了其对与营养细胞有丝分裂核分裂相关的碳源感应的影响。我们表明，在野生型或 plcA 缺陷型突变体中，葡萄糖在早期营养分生孢子萌发（6-7 小时）期间不会影响核分裂率。只有在培养 8 小时后，突变株的核复制才会出现一些减少。然而，当在添加多聚半乳糖醛酸盐的培养基中培养时，野生型的核分裂率降低，而我们的 plcA 缺陷型突变体在生长在这种碳源上时并没有表现出缓慢的核复制率，尽管它需要诱导和分泌多种果胶酶才能被代谢。因此，plcA 似乎直接与高分子量碳源感应相关。
