Ahn Chun-Seob, Oh YoungTaek, Kim Jeong-Geun, Han Kap-Hoon, Lee Chang-Won, Kim Jae Won
Department of Microbiology, College of Natural Science/Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
J Microbiol. 2014 Jul;52(7):590-6. doi: 10.1007/s12275-014-3651-x. Epub 2014 Jun 28.
To know the function of the plcA gene, which encodes a putative phosphoinositide-specific phospholipase C, in a model filamentous fungus Aspergillus nidulans, it was disrupted thorough homologous recombination and examined. The germination rate of ΔplcA was reduced by approximately 65% and germination of ΔplcA at a lower temperature (25°C) was much slower than germination under normal conditions (37°C), suggesting the plcA is responsible for cold-sensitivity. The hyphal growth of ΔplcA was slightly reduced at 37°C and conspicuously reduced at 25°C. While germinating ΔplcA formed giant swollen spores, and generated short and thick hyphae. The results of the nuclear examination of ΔplcA showed nuclear division with missegregation, and the rate of nuclear division was lower than that of wild type at both 25°C and 37°C. The results of this study showed that plcA is localized to the nucleus through intracellular calcium signaling in A. nidulans. The abnormal nuclear division, resulting from plcA gene deletion, affects conidiation in asexual development. Taken together, these results suggested that plcA is required for normal vegetative growth, morphogenesis, conidiation, and nuclear division in A. nidulans.
为了解编码假定的磷酸肌醇特异性磷脂酶C的plcA基因在丝状真菌构巢曲霉模型中的功能，通过同源重组对其进行了破坏并进行了检测。ΔplcA的萌发率降低了约65%，且ΔplcA在较低温度（25°C）下的萌发比正常条件（37°C）下慢得多，这表明plcA与冷敏感性有关。ΔplcA的菌丝生长在37°C时略有降低，在25°C时显著降低。在萌发时，ΔplcA形成巨大的肿胀孢子，并产生短而粗的菌丝。对ΔplcA进行细胞核检测的结果显示核分裂存在错误分离，并且在25°C和37°C时核分裂速率均低于野生型。本研究结果表明，在构巢曲霉中，plcA通过细胞内钙信号定位于细胞核。plcA基因缺失导致的异常核分裂影响无性发育中的分生孢子形成。综上所述，这些结果表明plcA是构巢曲霉正常营养生长、形态发生、分生孢子形成和核分裂所必需的。
