患有炎性 stifle 关节炎及相关退行性颅交叉韧带断裂的犬类关节组织中的淋巴细胞群体

Lymphocyte populations in joint tissues from dogs with inflammatory stifle arthritis and associated degenerative cranial cruciate ligament rupture.

作者信息

Muir Peter, Kelly Jennifer L, Marvel Sarah Jane, Heinrich Daniel A, Schaefer Susan L, Manley Paul A, Tewari Kavita, Singh Anju, Suresh M, Hao Zhengling, Plisch Erin

机构信息

Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.

出版信息

Vet Surg. 2011 Aug;40(6):753-61. doi: 10.1111/j.1532-950X.2011.00867.x. Epub 2011 Jul 19.

DOI:10.1111/j.1532-950X.2011.00867.x

PMID:21770988

Abstract

OBJECTIVE

To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR).

STUDY DESIGN

Prospective clinical study.

ANIMALS

Dogs (n=25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL.

METHODS

Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21.

RESULTS

The proportions of CD4(+) T helper lymphocytes, CD8(+) cytotoxic T lymphocytes, and CD3(+) CD4(-) CD8(-) T lymphocytes were increased in synovium from dogs with CCLR compared with synovium from healthy Beagle dogs (P<.05). The proportion of CD3(+) CD4(-) CD8(-) T lymphocytes in synovial fluid was increased in dogs with CCLR compared with dogs with OA (P<.05). In dogs with CCLR, the proportion of CD3(+) CD4(-) CD8(-) T lymphocytes in synovial fluid was inversely correlated with radiographic arthritis (S(R) =-0.68, P<.005).

CONCLUSION

Lymphocytic inflammation of stifle synovium and synovial fluid is an important feature of the CCLR arthropathy. Lymphocyte populations include T lymphocytes expressing CD4 and CD8, and CD3(+) CD4(-) CD8(-) T lymphocytes. Presence of CD3(+) CD4(-) CD8(-) T lymphocytes was associated with development of stifle synovitis. Further work is needed to fully identify the phenotype of these cells.

摘要

目的

评估患有退行性颅交叉韧带断裂（CCLR）的犬的膝关节滑膜和滑液中的淋巴细胞群。

研究设计

前瞻性临床研究。

动物

患有膝关节关节炎和CCLR的犬（n = 25）、7只患有与软骨退变相关关节炎（骨关节炎[OA]）的犬以及12只颅交叉韧带完整的健康比格犬。

方法

对CCLR犬的关节炎进行放射学分级。采集关节组织后，分离单核细胞，随后使用流式细胞术分析CD3、CD4、CD8和CD21的表达。

结果

与健康比格犬的滑膜相比，CCLR犬滑膜中CD4(+)辅助性T淋巴细胞、CD8(+)细胞毒性T淋巴细胞和CD3(+) CD4(-) CD8(-) T淋巴细胞的比例增加（P<0.05）。与OA犬相比，CCLR犬滑液中CD3(+) CD4(-) CD8(-) T淋巴细胞的比例增加（P<0.05）。在CCLR犬中，滑液中CD3(+) CD4(-) CD8(-) T淋巴细胞的比例与放射学关节炎呈负相关（S(R)= -0.68，P<0.005）。