Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Vet Microbiol. 2011 Mar 24;148(2-4):308-16. doi: 10.1016/j.vetmic.2010.09.011. Epub 2010 Sep 17.
It has been proposed that small quantities of microbial material within synovial joints may act as a trigger for development of synovitis. We have previously identified an association between intra-articular bacteria and development of inflammatory stifle arthritis and cranial cruciate ligament rupture (CCLR) in dogs, and now wished to quantify bacterial load and markers of synovitis in dogs with and without stifle arthritis and CCLR. Joint tissues were collected from dogs with CCLR (n=51) and healthy dogs with normal stifles (n=9). Arthritis was assessed radiographically in CCLR dogs. Bacterial load was assessed using qPCR and broad-ranging 16S rRNA primers. qRT-PCR was used to estimate expression of the T lymphocyte antigen receptor (TCR Vβ), CD3ɛ, tartrate-resistant acid phosphatase (TRAP), IL-4, IL-17, and TNF-α genes. Severity of synovitis was assessed histologically. Bacterial load was increased in arthritic stifles, when compared with healthy stifles. Histologic synovitis in arthritic stifles was mononuclear and was significantly correlated with bacterial load (1 of 2 primer sets) (S(R)=0.49, p<0.001). In arthritic stifles, expression of TRAP in synovium was increased relative to healthy stifles. Expression of pro-inflammatory genes was not correlated with bacterial load, histologic inflammation, or radiographic arthritis. Translocation of bacterial material to the canine stifle is related to the presence of joint inflammation. The lack of a strong positive correlation suggests that bacterial load is unlikely to be a primary pro-inflammatory factor. However, dysregulation of immune responses within synovial tissues may be dependent upon an environmental microbial trigger.
已经有人提出，关节内的少量微生物物质可能作为引发滑膜炎发展的触发因素。我们之前已经发现，关节内细菌与犬的炎症性膝关节关节炎和十字韧带断裂（CCLR）的发展之间存在关联，现在我们希望定量分析患有和不患有膝关节关节炎和 CCLR 的犬的关节内细菌负荷和滑膜炎标志物。从患有 CCLR 的犬（n=51）和健康的正常膝关节犬（n=9）中收集关节组织。在 CCLR 犬中通过放射学评估关节炎。使用 qPCR 和广泛的 16S rRNA 引物评估细菌负荷。使用 qRT-PCR 估计 T 淋巴细胞抗原受体（TCR Vβ）、CD3ɛ、抗酒石酸酸性磷酸酶（TRAP）、IL-4、IL-17 和 TNF-α 基因的表达。通过组织学评估滑膜炎的严重程度。与健康膝关节相比，关节炎膝关节的细菌负荷增加。关节炎膝关节的组织学滑膜炎为单核细胞，与细菌负荷（2 个引物组中的 1 个）呈显著相关（S(R)=0.49，p<0.001）。在关节炎膝关节中，滑膜中 TRAP 的表达相对于健康膝关节增加。促炎基因的表达与细菌负荷、组织学炎症或放射学关节炎无关。细菌物质向犬膝关节的易位与关节炎症的存在有关。缺乏强烈的正相关表明，细菌负荷不太可能是主要的促炎因素。然而，滑膜组织中免疫反应的失调可能取决于环境微生物触发因素。
