Department of Microbiology, Immunology, and Pathology, 1682 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USA.
Virol J. 2011 Jul 19;8:356. doi: 10.1186/1743-422X-8-356.
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8) plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.
我们开发了一种从转染哺乳动物细胞的质粒中生产传染性双亚基因组甲病毒的新方法。双亚基因组辛德毕斯病毒（TE3'2J）由巨细胞病毒 PolII 启动子转录，从而产生传染性病毒。转染低至 125ng 的质粒，在转染后 48 小时即可产生 1×10(8)噬斑形成单位/毫升（PFU/ml）的传染性病毒。该系统代表了生产重组辛德毕斯病毒的更有效方法。
