Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, India.
Asian Pac J Trop Med. 2011 Apr;4(4):310-4. doi: 10.1016/S1995-7645(11)60092-8. Epub 2011 May 29.
To search for new bioactive compounds from marine mollusks Melo melo (M. melo).
Preliminary work for bioactive compound was identified by using disc diffusion methods against human pathogens. Further analyses of compound were done by using TLC, SDS-PAGE. And also estimate the amount of protein in the samples by following Biuret method.
In antibacterial activity the maximum diameter of 24 mm zone of inhibition was recorded against Klebsiella pneumoniae (K. pneumoniae) strain of the mucus extract and minimum zone of inhibition of 11 mm was observed in Salmonella typhi (S. typhi) strain of body tissue extract. The antifungal activity of the extraction shows maximum activity against Trichophyton mentagarophytes (T. mentagarophytes) (14 mm) and minimum activity was recorded in Aspergillus flavus (A. flavus) (11 mm). The extract of mucus, nerve tissue, body tissue and kidney that showed antimicrobial activity was subjected to TLC to determine the presence of the peptides and amide groups, and also subjected to SDS-PAGE to estimate the molecular weight of proteins in a clear band were detected in the gel that represented kidney, body tissue, brain and mucus represent 14, 17, 22, 45 kDa.
The extracts from marine mollusks M. melo is the potential source of producing bioactive compounds against human pathogens and can be used for synthesis of new drugs.
从海洋软体动物芋螺（Melo melo）中寻找新的生物活性化合物。
采用圆盘扩散法对抗人体病原体，初步确定生物活性化合物；采用 TLC、SDS-PAGE 进一步分析化合物；并采用比色法测定样品中的蛋白质含量。
在抗菌活性方面，黏液提取物对肺炎克雷伯菌（K. pneumoniae）菌株的抑菌圈直径最大为 24mm，而体组织提取物对伤寒沙门氏菌（S. typhi）菌株的抑菌圈直径最小为 11mm。提取物的抗真菌活性对须癣毛癣菌（T. mentagarophytes）（14mm）的活性最大，而对黄曲霉（A. flavus）（11mm）的活性最小。显示抗菌活性的黏液、神经组织、体组织和肾脏提取物进行 TLC 以确定肽和酰胺基团的存在，并进行 SDS-PAGE 以估计凝胶中蛋白质的分子量，在凝胶中检测到清晰带，代表肾脏、体组织、脑和黏液分别为 14、17、22、45kDa。
海洋软体动物芋螺的提取物是产生抗人体病原体生物活性化合物的潜在来源，可用于合成新药。
