德国蜱传脑炎（TBE）流行地区自然暴露猴（Macaca sylvanus）和绵羊的 TBE 血清流行率以及蜱中的 TBE 病毒流行率。
Seroprevalence of tick-borne encephalitis (TBE) in naturally exposed monkeys (Macaca sylvanus) and sheep and prevalence of TBE virus in ticks in a TBE endemic area in Germany.
机构信息
Friedrich-Loeffler-Institute, Jena and Greifswald-Insel Riems, Germany.
出版信息
Ticks Tick Borne Dis. 2010 Sep;1(3):141-4. doi: 10.1016/j.ttbdis.2010.06.001. Epub 2010 Aug 13.
In a recently published study, a clinical case of severe tick-borne encephalitis (TBE) in a monkey (Macaca sylvanus) was described after natural exposure (tick bite) in a TBE endemic area in Germany, and from the tissue of this monkey the strain 'Salem', closely related to the strain 'Neudoerfl', was isolated and characterized. In this study, it was our aim to test all the available data for characterizing a TBE endemic area like TBE cases in humans and animals, TBE virus (TBEV) in ticks, and TBE-positive sera from animals. Sera from 283 monkeys and 100 sheep as well as 294 unfed ticks were collected at the monkey mountain Salem and its surroundings. A seroprevalence of 2.6% in monkeys and 9% in sheep were found. Furthermore, a new real-time RT-PCR method was established and used, in combination with an already published RT-qPCR, for TBEV genome detection in field-collected ticks, but no TBEV could be detected in the ticks tested.
在最近发表的一项研究中，描述了在德国 TBE 流行地区，一只猴子（Macaca sylvanus）在自然暴露（蜱叮咬）后发生严重蜱传脑炎（TBE）的临床病例，并从这只猴子的组织中分离出了与‘Neudoerfl’株密切相关的‘Salem’株。在这项研究中，我们的目的是测试所有可用的数据，以对 TBE 流行地区进行特征描述，如人类和动物中的 TBE 病例、蜱中的 TBE 病毒（TBEV）以及动物的 TBE 阳性血清。在萨勒姆猴山及其周边地区收集了 283 只猴子和 100 只绵羊的血清以及 294 只未吸血的蜱。在猴子中发现了 2.6%的血清阳性率，在绵羊中发现了 9%的血清阳性率。此外，还建立并使用了一种新的实时 RT-PCR 方法，结合已发表的 RT-qPCR，用于检测现场采集的蜱中的 TBEV 基因组，但在测试的蜱中未检测到 TBEV。
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