Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institute, Jena, Germany.
Clin Microbiol Infect. 2010 Mar;16(3):238-44. doi: 10.1111/j.1469-0691.2009.02764.x. Epub 2009 Nov 10.
Tick-borne encephalitis (TBE) is recognized as the most important viral tick-borne zoonosis in 27 countries in Europe. In this study, ticks were collected in Germany from two non-risk areas in the states of Saxony-Anhalt and Mecklenburg-Western Pomerania, where several single human TBE cases have occurred in recent years. Ticks were also collected from a region in Thuringia, known to be a former risk area for TBE virus (TBEV), where numerous human cases were reported between 1960 and 1975. Detection of TBEV RNA was conducted by real-time RT-PCR. No TBEV was detected in any field-collected ticks. However, ticks were also collected from volunteers living in Bavaria. Three of 239 ticks from this collection were positive for TBEV genome and two genetically distinct TBEV strains were detected and characterized.
蜱传脑炎（TBE）被认为是欧洲 27 个国家最重要的病毒性蜱传动物传染病。在这项研究中，从德国萨克森-安哈特州和梅克伦堡-前波美拉尼亚州的两个非风险地区采集了蜱虫，近年来这些地区发生了几例人类 TBE 病例。还从图林根州的一个地区采集了蜱虫，该地区已知是 TBE 病毒（TBEV）的前疫区，1960 年至 1975 年间报告了大量人类病例。通过实时 RT-PCR 检测 TBEV RNA。在任何野外采集的蜱虫中均未检测到 TBEV。然而，也从居住在巴伐利亚的志愿者身上采集了蜱虫。从该采集样本中，有 239 只蜱虫中的 3 只对 TBEV 基因组呈阳性，并且检测到并鉴定了两种具有不同遗传特征的 TBEV 株。
