利用 ISSR 标记研究印度致倦库蚊的种群遗传结构。

Population genetic structure of Culex quinquefasciatus in India by ISSR marker.

机构信息

Entomology Division, Defence R & D Establishment, Gwalior, Madhya Pradesh, India.

出版信息

Asian Pac J Trop Med. 2011 May;4(5):357-62. doi: 10.1016/S1995-7645(11)60103-X. Epub 2011 Jun 22.

DOI:10.1016/S1995-7645(11)60103-X

PMID:21771676

Abstract

OBJECTIVE

To characterize the genetic structure of various populations of Culex quinquefasciatus (Cx. quinquefasciatus) from India representing different geoclimatic locations.

METHODS

Inter simple sequence repeat (ISSR) markers were used. A set of 20 primers were screened with the laboratory populations of mosquito species. Finally the IS 40 primer was chosen based on the scorable banding pattern showing 100 percent polymorphism among the various populations. The statistical analysis was done using POPGENE 1.31 software. The consensus tree was generated based on UPGMA modified from NEIGHBOR procedure of PHYLIP Version 3.5.

RESULTS

The cluster analysis shows the main cluster which is divided into two sub cluster representing all the populations separated as per their phylogeographic and geoclimatic condition.

CONCLUSIONS

The findings will be helpful in understanding the population variation under different ecological conditions and development of effective vector management strategies.

摘要

目的

分析来自印度不同地理区域的各种库蚊（Culex quinquefasciatus，Cx. quinquefasciatus）种群的遗传结构。

方法

使用简单重复序列间（ISSR）标记。对蚊种的实验室种群进行了 20 个引物的筛选。最终，根据可评分的带型选择了 IS 40 引物，该引物在各种种群中显示出 100%的多态性。使用 POPGENE 1.31 软件进行统计分析。共识树是根据 PHYLIP 版本 3.5 的 NEIGHBOR 程序从 UPGMA 修改生成的。