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Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco.阿根廷查科地区骚扰锥蝽的精细遗传结构
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Fine-scale genetic structure in populations of the Chagas' disease vector Triatoma infestans (Hemiptera, Reduvidae).恰加斯病病媒骚扰锥蝽（半翅目，猎蝽科）种群中的精细遗传结构
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Population genetic structure of Culex quinquefasciatus in India by ISSR marker.利用 ISSR 标记研究印度致倦库蚊的种群遗传结构。
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Genetic structure of Triatoma infestans populations in rural communities of Santiago del Estero, northern Argentina.阿根廷北部圣地亚哥-德尔埃斯特罗农村社区侵扰锥猎蝽种群的遗传结构
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Microsatellites reveal a high population structure in Triatoma infestans from Chuquisaca, Bolivia.微卫星揭示了玻利维亚丘基萨卡的布氏田鼠体内存在高度的种群结构。
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Macrogeographic and microgeographic genetic structure of the Chagas' disease vector Triatoma infestans (Hemiptera: Reduviidae) from Catamarca, Argentina.来自阿根廷卡塔马卡的恰加斯病传播媒介侵扰锥猎蝽（半翅目：猎蝽科）的宏观地理和微观地理遗传结构
Genetica. 2008 Jul;133(3):247-60. doi: 10.1007/s10709-007-9208-8. Epub 2007 Sep 21.

简单序列重复区间标记在恰加斯病媒介昆虫（半翅目，猎蝽科）种群分析中的应用

Application of Inter-Simple Sequence Repeat Markers in the Analysis of Populations of the Chagas Disease Vector (Hemiptera, Reduviidae).

作者信息

Pérez de Rosas Alicia R, Restelli María F, Fernández Cintia J, Blariza María J, García Beatriz A

机构信息

Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.

出版信息

Am J Trop Med Hyg. 2017 Mar;96(3):660-663. doi: 10.4269/ajtmh.16-0717. Epub 2017 Apr 6.

DOI:10.4269/ajtmh.16-0717
PMID:28115670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5361542/
Abstract

Here we apply inter-simple sequence repeat (ISSR) markers to explore the fine-scale genetic structure and dispersal in populations of . Five selected primers from 30 primers were used to amplify ISSRs by polymerase chain reaction. A total of 90 polymorphic bands were detected across 134 individuals captured from 11 peridomestic sites from the locality of San Martín (Capayán Department, Catamarca Province, Argentina). Significant levels of genetic differentiation suggest limited gene flow among sampling sites. Spatial autocorrelation analysis confirms that dispersal occurs on the scale of ∼469 m, suggesting that insecticide spraying should be extended at least within a radius of ∼500 m around the infested area. Moreover, Bayesian clustering algorithms indicated genetic exchange among different sites analyzed, supporting the hypothesis of an important role of peridomestic structures in the process of reinfestation.

摘要

在此，我们应用简单序列重复区间（ISSR）标记来探究[具体物种]种群的精细尺度遗传结构和扩散情况。从30个引物中选取了5个引物，通过聚合酶链式反应扩增ISSR。在从阿根廷卡塔马卡省卡帕扬县圣马丁地区的11个住宅周边地点捕获的134个个体中，共检测到90个多态性条带。显著水平的遗传分化表明采样地点之间的基因流动有限。空间自相关分析证实扩散发生在约469米的尺度上，这表明杀虫剂喷洒应至少在受侵染区域周围约500米的半径范围内进行。此外，贝叶斯聚类算法表明所分析的不同地点之间存在基因交换，支持了住宅周边结构在再次侵染过程中起重要作用的假说。