Evidence for the biosynthesis of ceramide-phosphoethanolamine in brain synaptic plasma membrane vesicles and in sciatic nerve microsomes from normal and Trembler mice.

Synaptic plasma membrane vesicles (SPMV) from brains of normal and Trembler mice synthesized ceramide-phosphoethanolamine, and analogue of sphingomyelin from phosphatidylethanolamine (PE) and ceramide. The rate of this synthesis (6 nmol/mg of protein/h) and the (Na+)-K+)ATPase activity (about 70 mumol Pi per mg of protein/h) were very similar in normal and Trembler. The synthesis increased as a linear function of protein when endogenous PE was taken into account, but the addition of exogenous ceramide was without effect. Likewise, PE was the donor of phosphoethanolamine for the synthesis of the ceramide-phosphoethanolamine in normal and Trembler mouse sciatic nerves and this synthesis was 3.5 times greater in the mutant than in controls.