INRA, UMR 1318, Institut Jean-Pierre Bourgin, Route de Saint Cyr, 78026 Versailles, France.
J Cell Sci. 2011 Aug 15;124(Pt 16):2687-91. doi: 10.1242/jcs.088229. Epub 2011 Jul 19.
Two distinct pathways for meiotic crossover formation coexist in most eukaryotes. The Arabidopsis SHOC1 protein is required for class I crossovers and shows sequence similarity with the XPF endonuclease family. Active XPF endonucleases form a heterodimer with ERCC1 proteins. Here, we show that PTD, an ERCC1-like protein, is required for class-I-interfering crossovers along with SHOC1, MSH4, MSH5, MER3 and MLH3. SHOC1 interacts with PTD in a two-hybrid assay, through its XPF-like nuclease-(HhH)(2) domain. We propose that a XPF-ERCC1-like heterodimer, represented by SHOC1 and PTD in Arabidopsis, involving Zip2 in Saccharomyces cerevisiae and C9orf84 in human, is required for formation of class I crossovers.
两种不同的减数分裂交叉形成途径共存于大多数真核生物中。拟南芥 SHOC1 蛋白是类 I 交叉所必需的，与 XPF 内切酶家族具有序列相似性。活性 XPF 内切酶与 ERCC1 蛋白形成异二聚体。在这里，我们表明 PTD，一种 ERCC1 样蛋白，与 SHOC1、MSH4、MSH5、MER3 和 MLH3 一起，需要类-I-干扰交叉。在双杂交测定中，SHOC1 通过其 XPF 样核酸内切酶-(HhH)(2)结构域与 PTD 相互作用。我们提出，拟南芥中的 XPF-ERCC1 样异二聚体（由 SHOC1 和 PTD 代表），涉及酿酒酵母中的 Zip2 和人类中的 C9orf84，是形成类 I 交叉所必需的。
