Institut für Organische und Biomolekulare Chemie, Tammannstr. 2, 37077 Göttingen, Germany.
Chem Commun (Camb). 2011 Sep 7;47(33):9405-7. doi: 10.1039/c1cc12879e. Epub 2011 Jul 20.
SNARE proteins mediate membrane fusion between synaptic vesicles and the plasma membrane. A minimized peptide SNARE model system with reduced complexity was introduced combining the native SNARE transmembrane (TMD) and linker domains with artificial coiled-coil forming peptides. Specific membrane fusion initiated by coiled-coil recognition was shown by lipid and content mixing vesicle assays.
SNARE 蛋白介导突触小泡与质膜之间的膜融合。引入了一种简化的 SNARE 肽模型系统，该系统具有简化的复杂性，将天然 SNARE 跨膜（TMD）和连接域与人工卷曲螺旋形成肽结合在一起。通过脂质和内容混合囊泡测定显示，卷曲螺旋识别可特异性引发膜融合。
