Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, Wuhan, People's Republic of China.
Fish Physiol Biochem. 2012 Apr;38(2):585-93. doi: 10.1007/s10695-011-9540-x. Epub 2011 Jul 20.
Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.
组蛋白去乙酰化酶 4（HDAC4）是一种重要的染色质重塑修饰酶，在调节基因表达中起着至关重要的作用。时空表达谱显示，斑马鱼 hdac4 mRNA 在各种组织中广泛分布，在 36 hpf（受精后小时）和 6 dpf（受精后天）的表达水平明显高于其他时期。曲古抑菌素 A（TSA）抑制斑马鱼胚胎的发育以及 hdac4 和 mef2a（肌细胞增强因子-2A）的转录。此外，构建了含有不同 hdac4 启动子区域的 5 个载体，以分析启动子活性。含有-125 至+160 区域的载体在两种细胞中的荧光素酶活性分别比对照高约 30.3 倍和 58.9 倍。通过比较-302 至+30 区域和-698 至+30 区域的荧光素酶活性，表明-698 至-302 区域可能含有温和的负调控元件。
