The Chicken Promoter and Its Regulation by MYC and HIF1A.

BACKGROUND

Histone deacetylase 4 () is a member of the class II histone deacetylase family, whose members play a crucial role in various biological processes. An in-depth investigation of the transcriptional characteristics of chicken can provide fundamental insights into its function.

METHODS

We examined expression in chicken embryonic stem cells (ESC) and spermatogonial stem cells (SSC) and cloned a 444 bp fragment from upstream of the chicken transcription start site. Subsequently, we constructed pEGFP-HDAC4 and a series of 5'-deletion luciferase reporter constructs, which we transfected into DF-1 cells to measure their transcriptional activity. The regulatory mechanisms of chicken expression were investigated by performing trichostatin A (TSA) treatment, deleting putative transcription factor binding sites, and altering transcription factor expression levels.

RESULTS

exhibited higher expression in SSC than in ESC. We confirmed that the upstream region from -295 bp to 0 bp is the core transcriptional region of . TSA effectively inhibited transcription, and bioinformatics analysis indicated that the chicken core promoter sequence exhibits high homology with those of other avian species. The myelocytomatosis viral oncogene homolog (MYC) and hypoxia-inducible factor 1 α (HIF1A) transcription factors were predicted to bind to this core region. Treatment with TSA for 24 h resulted in the upregulation of MYC and HIF1A, which repressed transcription.

CONCLUSIONS

Our results provide a basis for subsequent investigations into the regulation of expression and biological function.