Department of Anthropology, University of North Carolina at Chapel Hill, USA.
Am J Hum Biol. 2011 Nov-Dec;23(6):820-2. doi: 10.1002/ajhb.21205. Epub 2011 Jul 19.
This study reports the validation of a noninvasive method for repeated assessment of testosterone from infant fecal samples.
Fecal samples were collected from cotton diaper liners, subjected to methanol extraction, and assayed using a modified commercial testosterone RIA kit.
Method validity was supported by a recovery near 100%, a sensitivity of 1.23 pg/ml, and inter- and intra-assay coefficients of variations less than 10 and 15%, respectively. Testosterone was detected in all samples from male and female infants aged 2 weeks to 15 months.
Fecal assessment is supported as a novel, non-invasive tool for studying testosterone during early human development.
本研究报告了一种从婴儿粪便样本中重复评估睾酮的非侵入性方法的验证。
从棉质尿布衬垫中收集粪便样本，进行甲醇提取，并使用改良的商业睾酮 RIA 试剂盒进行检测。
该方法的有效性得到了回收率接近 100%、灵敏度为 1.23pg/ml 以及批内和批间变异系数分别小于 10%和 15%的支持。从 2 周到 15 个月大的男婴和女婴的所有粪便样本中均检测到了睾酮。
粪便评估支持作为研究人类早期发育过程中睾酮的一种新颖的非侵入性工具。
