Witherow D Scott, Carson Sue
Department of Plant Biology, Biotechnology Program, North Carolina State University, Raleigh, North Carolina 27695, USA.
Biochem Mol Biol Educ. 2011 Jul;39(4):300-8. doi: 10.1002/bmb.20506.
The study of protein-protein interactions is important to scientists in a wide range of disciplines. We present here the assessment of a lab-intensive course that teaches students techniques used to identify and further study protein-protein interactions. One of the unique elements of the course is that students perform a yeast two-hybrid screen and identify novel protein-protein interactions in what is essentially the beginning of an independent research project in the context of a class. While students benefit from the research-like experience, data is actively generated that can be further studied in independent research projects. Student learning outcomes were assessed using a questionnaire that was given to students before and after the course. The results indicate that students' conceptual and technical understanding of the methodologies taught in the class increased, and that hands-on experience in the lab was perceived to be the most important component of the course.
蛋白质-蛋白质相互作用的研究对众多学科的科学家来说都很重要。我们在此展示了一门实验密集型课程的评估情况，该课程教授学生用于识别和进一步研究蛋白质-蛋白质相互作用的技术。该课程的独特之处之一在于，学生要进行酵母双杂交筛选，并在课堂背景下的一个独立研究项目伊始识别新的蛋白质-蛋白质相互作用。虽然学生从类似研究的经历中受益，但同时也积极生成了可在独立研究项目中进一步研究的数据。通过在课程前后向学生发放问卷来评估学生的学习成果。结果表明，学生对课堂所教授方法的概念性和技术性理解有所增强，并且实验室的实践经验被认为是该课程最重要的组成部分。
