Histology and Embryology Department, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
J Tissue Eng Regen Med. 2011 Aug;5(8):612-9. doi: 10.1002/term.353. Epub 2010 Dec 29.
Topical application of the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) is known to exert beneficial effects on wound healing. The aim of this study was to evaluate, for the first time, the effect of topical application of GSNO on the healing of ischaemic wounds. Wistar rats were submitted to two parallels incisions on their backs; the skin was separated from the underlying tissue, the incisions were sutured and an excisional wound was made between the parallel incisions to create an ischaemic condition surrounding the wound. The animals were separated into a control group, which received a hydrogel vehicle without GSNO, and a GSNO-treated group, which received a GSNO-containing hydrogel. The animals were treated for 7 days consecutively with one daily application. The GSNO-treated group displayed higher rates of wound contraction and re-epithelization, lower amounts of inflammatory cells, an increase in collagen fibre density and organization and a decrease in the neovascularization compared to control. These results show that topical application of GSNO is effective in the treatment of ischaemic wounds, leading to a significant improvement in the wound healing. Therefore, topical GSNO-containing hydrogels have potential for the therapeutic treatment of ischaemic diabetic and venous ulcers.
局部应用一氧化氮（NO）供体 S-亚硝基谷胱甘肽（GSNO）已被证实对伤口愈合有有益的影响。本研究的目的是首次评估局部应用 GSNO 对缺血性伤口愈合的影响。Wistar 大鼠背部做两个平行切口；将皮肤与下面的组织分离，缝合切口，并在平行切口之间做一个切除性伤口，以在伤口周围形成缺血条件。动物分为对照组（给予不含 GSNO 的水凝胶载体）和 GSNO 处理组（给予含 GSNO 的水凝胶）。动物连续治疗 7 天，每天一次。与对照组相比，GSNO 处理组的伤口收缩和再上皮化速度更快，炎症细胞数量更少，胶原纤维密度和组织增加，新生血管减少。这些结果表明，局部应用 GSNO 可有效治疗缺血性伤口，显著改善伤口愈合。因此，局部含有 GSNO 的水凝胶具有治疗缺血性糖尿病和静脉溃疡的潜力。
